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Intermolecular interactions in albumin-sulfoxide-water systems at low temperatures, investigated by means of fluorescence quenching

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Abstract

The effect of low temperatures on the interaction in human serum albumin (HSA)-diethyl sulfoxife (DESO)-dipropyl sulfoxide (DPSO)-water systems is investigated by means of fluorescence spectroscopy (intrinsic protein fluorescence, three-dimensional excitation/emission matrix (3D EEM)). The Stern-Volmer constants of HSA quenching are calculated for these systems. The structural changes occurring in these systems are characterized using 3D EEM profiles of HSA. It is shown that the HSA structural changes depend not only on the direct interaction of protein with sulfoxides but on structural changes in the solvent as well.

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Authors and Affiliations

  1. Yerevan State University, Yerevan, 0025, Armenia

    K. R. Grigoryan & H. A. Shilajyan

Authors
  1. K. R. Grigoryan
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  2. H. A. Shilajyan
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Correspondence to K. R. Grigoryan.

Additional information

Original Russian Text © K.R. Grigoryan, H.A. Shilajyan, 2013, published in Zhurnal Fizicheskoi Khimii, 2013, Vol. 87, No. 5, pp. 800–803.

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Grigoryan, K.R., Shilajyan, H.A. Intermolecular interactions in albumin-sulfoxide-water systems at low temperatures, investigated by means of fluorescence quenching. Russ. J. Phys. Chem. 87, 780–782 (2013). https://doi.org/10.1134/S0036024413050099

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  • DOI: https://doi.org/10.1134/S0036024413050099

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