Abstract
A series of polystyrene standards was separated by means of hydrodynamic chromatography on a open capillary with a length of 5 m (active zone is 4.5 m) and an inner diameter of 2 μm. Unusually high efficiency in the separation of polymers with masses of up to 500 kDa was noticed: 1.5 × 106 theoretical plates at a retention time of ∼70 min for a sample of polystyrene with a mass of 390 kDa. It was established that the column efficiency decreases rapidly for polymers with a mass above 500 kDa. A calibration curve for polystyrene standards was constructed. It was shown that, in contrast to the models described in the literature, it is described by a simple two-term expression without quadratic terms. It is concluded that hydrodynamic chromatography is a technique complementary to exclusion chromatography, especially in the separation of high-molecular polymers.
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Original Russian Text © A.A. Korolev, E.N. Viktorova, V.A. Orekhov, A.A. Kurganov, 2012, published in Zhurnal Fizicheskoi Khimii, 2012, Vol. 86, No. 7, pp. 1280–1284.
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Korolev, A.A., Viktorova, E.N., Orekhov, V.A. et al. Unusually high efficiency of the separation of polymers by hydrodynamic chromatography on hollow capillary columns. Russ. J. Phys. Chem. 86, 1161–1164 (2012). https://doi.org/10.1134/S0036024412070096
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DOI: https://doi.org/10.1134/S0036024412070096