Abstract
The newly established instrumentation of HPLC/DAD, FTIR, and NMR techniques have been applied for simultaneous identification and physicochemical compatibility determination of the potential major antiseptic constituents (Hypericum perforatum L. and Usnea barbata extracts) which can be present in some new origin pharmaceutical preparation. Based on the obtained results the conclusion is that a simultaneous use of the analyzed constituents in production of some new preparations with antiseptic properties is possible. The chromatographic separation of antiseptic mixture was performed on a RP-HPLC C18 column. For the NMR detection, the analytes eluted from LC column were trapped and hereafter transported into the NMR flow-cell. The NMR and FTIR techniques allowed the characterization of the major constituent of Hypericum perforatum L., mainly hypericin, as well as of Usnea barbata, mainly usnic acid.
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Nikolić, G.S., Zlatković, S. & Nikolić, N. Identification and compatibility of the major active principles in some new natural origin antiseptics. Russ. J. Phys. Chem. 83, 1612–1616 (2009). https://doi.org/10.1134/S0036024409090350
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DOI: https://doi.org/10.1134/S0036024409090350