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A fullerene C60-based ligand in a stationary phase for affine chromatography of membrane porphyrin-binding proteins

  • Biophysical Chemistry
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Abstract

A new affine chromatography technique is suggested for the purification of porphyrin-binding proteins (PBP) from mammal cell membranes. The procedure uses new fullerene-porphyrin ligands immobilized on agarose and bound to the polysaccharide matrix via the epoxycyclohexyl residue. A selective PBP stationary phase was used in a single-column chromatography run for the complete purification of a monomeric protein (17.6 kDa) from mitochondrial membranes of rat myocardium. This protein was characterized by high affinity for porphyrin-related structures. To separate it from other nonspecifically sorbed membrane proteins, synchronous linear pH and ionic strength gradients were used.

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Authors and Affiliations

  1. Sechenov Medical Academy, Moscow, Russia

    N. Amirshakhi & R. N. Alyautdin

  2. Faculty of Chemistry, Moscow State University, Moscow, Russia

    A. P. Orlov & A. A. Poloznikov

  3. Semenov Institute of Chemical Physics, Russian Academy of Sciences, ul. Kosygina 4, Moscow, Russia

    D. A. Kuznetsov

Authors
  1. N. Amirshakhi
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  2. R. N. Alyautdin
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  3. A. P. Orlov
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  4. A. A. Poloznikov
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  5. D. A. Kuznetsov
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Corresponding author

Correspondence to N. Amirshakhi.

Additional information

Original Russian Text © N. Amirshakhi, R.N. Alyautdin, A.P. Orlov, A.A. Poloznikov, D.A. Kuznetsov, 2008, published in Zhurnal Fizicheskoi Khimii, 2008, Vol. 82, No. 11, pp. 2170–2175.

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Amirshakhi, N., Alyautdin, R.N., Orlov, A.P. et al. A fullerene C60-based ligand in a stationary phase for affine chromatography of membrane porphyrin-binding proteins. Russ. J. Phys. Chem. 82, 1952–1957 (2008). https://doi.org/10.1134/S0036024408110277

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  • DOI: https://doi.org/10.1134/S0036024408110277

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