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Distribution or adsorption: the major dilemma in reversed-phase HPLC

  • Presented at the Conference “Chromatography in Chemical Analysis and Physicochemical Investigations”
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Abstract

A method is suggested for analyzing the dependences obtained for different compositions of mobile eluent system phases, their slopes and intercepts, logk(i, B) = a + b logk (i, A), where a is the intercept for the A and B stationary phases and b is the proportionality factor. An analysis requires parallel investigation of sorbate retention on at least three stationary phases with different lengths of grafted hydrocarbon radicals. The dependence of correlation parameters on the sorbate retention mechanism is discussed. It is shown that the hypothetical dependences coincide with the experimental dependences for surface sorption of resveratrol and volume distribution of triglycerides.

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Authors and Affiliations

  1. Belgorod State University, Belgorod, Russia

    V. I. Deineka

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  1. V. I. Deineka
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Correspondence to V. I. Deineka.

Additional information

Original Russian Text © V.I. Deineka, 2008, published in Zhurnal Fizicheskoi Khimii, 2008, Vol. 82, No. 6, pp. 1028–1032.

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Deineka, V.I. Distribution or adsorption: the major dilemma in reversed-phase HPLC. Russ. J. Phys. Chem. 82, 902–905 (2008). https://doi.org/10.1134/S0036024408060046

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  • DOI: https://doi.org/10.1134/S0036024408060046

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