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Comparative Study of Biochemical and Histopathological Parameters of Two Black Sea Goby Species

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The aim of the present study was to conduct the comparative analysis of the set of biochemical and histopathological parameters of the goby Neogobius melanostomus (Pallas, 1814) and the knout goby Mesogobius batrachocephalus (Pallas, 1814) collected from Apollonova Bay (the Black Sea, Sevastopol). The biochemical analyses performed in the tissues of two fish species revealed shifting in the prooxidant-antioxidant reactions towards the lipid peroxidation processes in liver, illustrated decreased activity of alanine aminotransferase and alkaline phosphatase in the organ and its increase in serum of the round goby, thus showing more pronounced oxidative damages of hepatocytes as compared to the knout goby and was confirmed histologically. Necrosis of single hepatocytes was detected in 44.44% of the round goby in contrast to 28% in the knout goby. The histopathological studies of gills, kidneys and liver revealed 22 alterations in the round goby and 18 in the knout goby, that belonged to five types of patterns: circulatory disorders, inflammatory reactions, progressive and regressive changes, benign tumours. In liver (lipoid vacuolization of hepatocytes) and kidney (vacuolization and hyaline-droplet degeneration of nephrocytes, nephrocalcinosis, local necrosis of the renal tubules) regressive changes dominated and were registered as much more common for the round goby. The most histopathological lesions in gobies’ gills (hyperplasia of the respiratory epithelium and fusion of the gill lamellae) were found progressive and related to the compensatory-adaptive reactions. Thus, the obtained results indicate a moderate chronic toxic effects of contaminants on fish in Apollonova Bay, and testify greater sensitivity of the round goby to the level of mixed pollution /Cu content in this water body, and hence make it more preferable for biodiagnostics throughout the water regions. Based on our researches, we can recommend the joint application of biochemical and histopathological parameters of two goby species to ensure the correct interpretation in bioindication studies.

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  1. SanPiN 2.3.2.1078-01. Hygienic requirements for the quality and safety of food raw materials and food products. Sanitary rules and regulations, 2002. https://base.garant.ru/4178234/. Version 02/ 2023.

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ACKNOWLEDGMENTS

We would like to thank to S.I. Perepelitsa (А.О. Kovalevsky Institute of Biology of the Southern Seas of RAS) for the delivery of ichthyological material from the study area.

Funding

This work was conducted under financial support of the Russian Academy of Science research grant № 1023032000049-6-1.6.21.

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  1. Kovalevsky Institute of Biology of the Southern Seas, Russian Academy of Science, Sevastopol, Russia

    T. V. Gavruseva & T. B. Sigacheva

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Animal-related experiments were conducted in accordance with the NIH Guidelines for the care and use of laboratory animals (http://oacu.od.nih.gov/regs/index.htm). Animal protocols were approved by the Bioethics Commission of Kovalevsky Institute of Biology of the Southern Seas of RAS, Sevastopol, Russia (Protocol No. 2/23 of August 24, 2023).

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Gavruseva, T.V., Sigacheva, T.B. Comparative Study of Biochemical and Histopathological Parameters of Two Black Sea Goby Species. J. Ichthyol. (2024). https://doi.org/10.1134/S0032945224700048

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