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A Comparative Study of the Leukocyte Formula of the Peripheral Blood, Head Kidney and Spleen of the Bream Abramis brama (Cyprinidae) in the Middle Volga Reservoirs

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Abstract

A comparative study of the leukocyte formula of peripheral blood, the head section of the kidney and spleen of the bream Abramis brama, inhabiting the reservoirs of the Middle Volga (Kuibyshev, Cheboksary and Gorky), was carried out. In the studied fish, a predominance of lymphocytes and a low content of neutrophils were recorded in the peripheral blood and spleen. In the head kidney of the breams from the Kuibyshev Reservoir, a significantly high level of blast forms of cells and a low level of neutrophils were noted, and in individuals from the Gorky Reservoir, a low content of monocytes and the absence of eosinophils were noted compared to fish from other reservoirs.

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Notes

  1. Approved by the Presidium of the Council under the President of the Russian Federation for Strategic Development and Priority Projects (Minutes no. 9 of August 30, 2017).

REFERENCES

  1. Abram, Q.H., Dixon, B., and Katzenback, B.A., Impacts of low temperature on teleost immune system, Biology, 2017, vol. 6, no. 4, Article 39. https://doi.org/10.3390/biology6040039

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  2. Atlas presnovodnykh ryb Rossii (Atlas of Freshwater Fishes of Russia), Moscow: Nauka, 2002, vol. 1.

  3. Balabanova, L.V., The fate of parenterally introduced bacteria in the body of fish, in Fiziologiya i parazitologiya presnovodnykh zhivotnykh (Physiology and Parasitology of Freshwater Animals), Leningrad: Nauka, 1979, pp. 88–104.

  4. Basova, M.M., White blood cell count of the scorpion fish Scorpaena porcus as a biomarker of anthropogenic pollution in the Black Sea coastal waters, J. Ichthyol., 2017, vol. 57, no. 3, pp. 467–472. https://doi.org/10.1134/S003294521703002X

    Article  Google Scholar 

  5. Ellis, A.E., The leucocytes of fish: a review, J. Fish. Biol., 1977, vol. 11, no. 5, pp. 453−491. https://doi.org/10.1111/j.1095-8649.1977.tb04140.x

    Article  Google Scholar 

  6. Fänge, R., A comparative study of limphomyeloid tissue in fish, Dev. Comp. Immunol., no. Suppl. 2, 1982, pp. 23–33.

  7. Gerasimov, Yu.V., Malin, M.I., Solomatin, Yu.I., et al., Distribution and structure of the fish population in the reservoirs of the Volga cascade in the 1980s and 2010s, Tr. Inst. Biol. Vnutr. Vod Ross. Akad. Nauk, 2018, no. 82 (85), pp. 82–106. https://doi.org/10.24411/0320-3557-2018-1-0014

  8. Golovanov, V.K., Temperature and fish health. Ecological, physiological, biochemical and immunological aspects, Mater. IV Mezhdunar. konf. “Problemy immunologii, patologii i okhrany zdorov’ya ryb” (Proc. IV Int. Conf. “Problems of Immunology, Pathology and Health Protection of Fish”), Yaroslavl: Filigran’, 2015, pp. 11–19. https://doi.org/10.13140/RG.2.1.4494.4084

  9. Golovanov, V.K. and Mikryakov, D.V., Modifying effect of temperature on fish immunity to infectious diseases, Mater. III Mezhdunar. konf. “Problemy immunologii, patologii i okhrany zdorov’ya ryb” (Proc. III Int. Conf. “Problems of Immunology, Pathology and Health Protection of Fish”). Moscow: Ross. Gos. Agrar. Univ.–Mosk. Sel’.-Khoz. Akad., 2011, pp. 95–99. https://doi.org/10.13140/RG.2.1.1223.0568

  10. Havixbeck, J.J. and Barreda, D.R., Neutrophil development, migration, and function in teleost fish, Biology, 2015, vol. 4, no. 4, pp. 715–734. https://doi.org/10.3390/biology4040715

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  11. Hodgkinson, J.W., Grayfer, L., and Belosevic, M., Biology of bony fish macrophages, Ibid., 2015, vol. 4, no. 4, pp. 881–906. https://doi.org/10.3390/biology4040881

    Article  CAS  Google Scholar 

  12. Ivanova, N.T., Atlas kletok krovi ryb (Atlas of Fish Blood Cells), Moscow: Leg. i Pishch. Prom-st’, 1983.

  13. Keister, I.A., Dynamics of fish blood parameters as an indicator of the state of the ichthyofauna of Lake Beloe (Vologda oblast), Zh. nauch. publikatsii aspirantov i doktorantov, 2007, no. 12, Version 03/2022. http://jurnal.org/articles/2007/bio6.html.

  14. Katzenback, B.A. and Belosevic, M., Isolation and functional characterization of neutrophil-like cells, from goldfish (Carassius auratus L.) kidney, Dev. Comp. Immunol., 2009, vol. 33, no. 4, pp. 601–611. https://doi.org/10.1016/j.dci.2008.10.011

    Article  CAS  PubMed  Google Scholar 

  15. Kondrat’eva, I.A., Kitashova A.A., and Lange, M.A., Modern ideas about the immune system of fish, Vestn. Mosk. Univ., 2001, vol. 16, no. 4, pp. 11–20.

    Google Scholar 

  16. Kuzina, T.V., Cytophysiological features of the blood of commercial fish of the Volga-Caspian Canal, Extended Abstract of Cand. Sci. (Biol.) Dissertation, Astrakhan State University, Astrakhan, 2011.

  17. Lapirova, T.B. and Flerova, E.A., Comparative analysis of indicators of the immunophysiological state of the bream Abramis brama (L.) from areas of the Rybinsk Reservoir with different degrees of pollution, Vestn. Michurin. Gos. Agrar. Univ., 2015, no. 2, pp. 83–88.

  18. Lapirova, T.B. and Zabotkina, E.A., Comparative analysis of the indices of immunophysiological state in bream (Abramis brama (L.)) from parts of the Rybinsk Reservoir with different extents of pollution, Inland Water Biol., 2010, vol. 3, no. 2, pp. 181–186.

    Article  Google Scholar 

  19. Lapirova, T.B. and Zabotkina, E.A., Study of the blood pattern of the bream (Abramis brama L.) at the first detection of fish infection with trypanosomes in the Uglich Reservoir, Tez. dokl. Vseros. konf. “Volga i ee zhizn’” (Abstr. All-Russ. Conf. “Volga and Its Life”), Yaroslavl: Filigran’, 2018, p. 85.

    Google Scholar 

  20. Le Morvan, C., Troutaud, D., and Deschaux, P., Differential effects of temperature on specific and nonspecific immune defences in fish, J. Exp. Biol., 1998, vol. 201, no. 2, pp. 165–168. https://doi.org/10.1242/jeb.201.2.165

    Article  CAS  PubMed  Google Scholar 

  21. Manning, M.J. and Nakanishi, T., The specific immune system: Cellular defenses, in The Fish Immune System: Organism, Pathogen and Environment, London: Acad. Press, 1996, pp. 160–206.

    Google Scholar 

  22. Mikryakov, V.R., Zakonomernosti formirovaniya priobretennogo immuniteta u ryb (Patterns of the Formation of Acquired Immunity in Fish), Rybinsk: Inst. Biol. Vnutr. Vod Ross. Akad. Nauk, 1991.

  23. Mikryakov, V.R. and Balabanova, L.V., Fundamentals of cellular immunity in fish, in Fiziologiya i parazitologiya presnovodnykh zhivotnykh (Physiology and Parasitology of Freshwater Animals) Leningrad: Nauka, 1979, pp. 105–124.

  24. Mikryakov, V.R. and Lapirova, T.B., Influence of salts of some heavy metals on the composition of the white blood of the juvenile Lena sturgeon Acipenser baeri, Vopr. Ikhtiol., 1997, vol. 37, no. 4, pp. 538–542.

    CAS  Google Scholar 

  25. Mikryakov, V.R., Balabanova, L.V., Zabotkina, E.A., et al., Reaktsiya immunnoi sistemy ryb na zagryaznenie vody toksikantami i zakislenie sredy (Response of the Immune System of Fish to Water Pollution by Toxicants and Acidification of the Environment), Moscow: Nauka, 2001.

  26. Mikryakov, V.R., Tereshchenko, V.G., and Mikryakov, D.V., Using the integral index to assess destabilization processes in the immune system of fish, Inland Water Biol., 2021, vol. 14, no. 3, pp. 340–348. https://doi.org/10.1134/S199508292103010X

    Article  Google Scholar 

  27. Mineev, A.K., Morphological analysis and pathological changes in the structure of blood cells in fishes from Saratov Reservoir, J. Ichthyol., 2007, vol. 47, no. 1, pp. 87–93.

    Article  Google Scholar 

  28. Mineev, A.K., Hematopathology in fish of the Kuibyshev Reservoir, Izv. Samar. Nauchn. Tsentra Ross. Akad. Nauk, 2016, vol. 18, no. 5, pp. 51–59.

    Google Scholar 

  29. Moiseenko, T.I., Vodnaya ekotoksikologiya (Aquatic Ecotoxicology), Moscow: Nauka, 2009.

  30. Scapigliati, G., Functional aspects of fish lymphocytes, Dev. Comp. Immunol., 2013, vol. 41, no. 2, pp. 200–208. https://doi.org/10.1016/j.dci.2013.05.012

    Article  CAS  PubMed  Google Scholar 

  31. Suvorova, T.A. and German, A.V., Composition of leukocytes in the bream of the Saratov Reservoir, Rybovodstvo i Rybn. Khoz-vo, 2021, no. 2, pp. 44–51. https://doi.org/10.33920/sel-09-2102-04

  32. Trofimova, L.V. and Mikryakov, V.R., Functioning of the immunological system of crucian carp at different temperatures, Inform. Byul. Inst. Biol. Vnutr. Vod Akad. Nauk SSSR, 1973, no. 19, pp. 44–47.

  33. Uribe, C., Folch, H., Enriquez, R., and Moran, G., Innate and adaptive immunity in teleost fish: A review, Vet. Med., 2011, vol. 56, no. 10, pp. 486–503. https://doi.org/10.17221/3294-VETMED

    Article  CAS  Google Scholar 

  34. Van Muiswinkel, W., The immune system: Effects of water temperature and acidification, in Climate Change and Non-Infectious Fish Disorders, Wallingford: CABI, 2020, pp. 218–236. https://doi.org/10.1079/9781786393982.0218

  35. Van Muiswinkel, W. and Vervoorn-Van Der Wal, B., The immune system of fish, in Fish Diseases and Disorders, Wallingford: CABI, 2006, vol. 1, pp. 678–701. https://doi.org/10.1079/9780851990156.0678

  36. Zabotkina, E.A., Serednyakov, V.E., Trofimov, D.Yu., et al., Ecological plasticity of hematological indicators of the bream in reservoirs of the Volga basin, Tez. dokl. Vseros. konf. “Volga i ee zhizn’” (Abstr. All-Russ. Conf. “Volga and Its Life”), Yaroslavl: Filigran’, 2018, p. 49.

    Google Scholar 

  37. Zapata, A.G., Chiba, A., and Varas, A., 1—Cells and tissues of the immune system of fish, Fish Physiol., 1996, vol. 15, pp. 1–62. https://doi.org/10.1016/S1546-5098(08)60271-X

    Article  Google Scholar 

  38. Zhiteneva, L.D., Makarov, E.V., and Rudnitskaya, O.A., Osnovy ikhtiogematologii (v sravnitel’nom aspekte) (Fundamentals of Ichthyohematology (In a Comparative Aspect)), Rostov-on-Don: Everest, 2004.

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Funding

The work was carried out within the framework of the state assignment no. 121050500046-8.

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Authors and Affiliations

  1. Papanin Institute for Biology of Inland Waters Russian Academy of Sciences, Borok, Nekouzskii raion, Yaroslavl oblast, Russia

    T. A. Suvorova, A. V. German & D. V. Mikryakov

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  1. T. A. Suvorova
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  2. A. V. German
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  3. D. V. Mikryakov
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Correspondence to T. A. Suvorova.

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Translated by S. Avodkova

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Suvorova, T.A., German, A.V. & Mikryakov, D.V. A Comparative Study of the Leukocyte Formula of the Peripheral Blood, Head Kidney and Spleen of the Bream Abramis brama (Cyprinidae) in the Middle Volga Reservoirs. J. Ichthyol. 63, 382–386 (2023). https://doi.org/10.1134/S0032945223010149

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