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Correction of Fluorescent Images of Biological Tissues for the Effects of Light Absorption and Scattering

  • Biophotonics
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Abstract

A method for correcting the influence of light attenuation processes in biological tissues on their fluorescent images is proposed. The transfer function that takes into account the radiation losses in the medium at the wavelengths of excitation and emission of fluorescence is calculated analytically, depending on the transport scattering index and the hemoglobin tissue index. The latter is determined on the basis of a color image of the tissue in reflected visible light. Verification of the developed method was carried out on the basis of computer simulation of fluorescent images of biological tissue by the Monte Carlo method.

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References

  1. N. Ramanujam, in Encyclopedia of Analytical Chemistry, Ed. by R. Meyers (Wiley, Chichester, 2000), p. 20. doi 10.1002/9780470027318.a0102

  2. V. V. Sokolov, I. G. Rusakov, N. N. Bulgakova, R. V. Ul’yanov, and A. A. Teplova, Sib. Onkol. Zh., No. 4 (24), 117 (2007).

    Google Scholar 

  3. A. A. Shiryaev, G. Kh. Musaev, M. V. Loshchenov, A. V. Borodkin, V. V. Levkin, N. L. Okhotnikova, V. V. Volkov, V. I. Makarov, and V. B. Loshchenov, Biomed. Photon. 5 (4), 15 (2016).

    Article  Google Scholar 

  4. J. Y. Qu, C. E. MacAulay, S. Lam, and B. Palcic, Opt. Eng. 34, 3334 (1995). doi 10.1117/12.212917

    Article  ADS  Google Scholar 

  5. G. I. Zonios, R. M. Cothren, J. T. Arendt, J. Wu, Van J. Dam, J. M. Crawford, R. Manoharan, and M. S. Feld, IEEE Trans. Biomed. Eng. 43, 113 (1996). doi 10.1109/10.481980

    Article  Google Scholar 

  6. B. Zhu and E. M. Sevick-Muraca, Br. J. Radiol. 88, 20140547 (2015). doi 10.1259/bjr.20140547

    Article  Google Scholar 

  7. P. A. Valdés, F. Leblond, V. L. Jacobs, B. C. Wilson, K. D. Paulsen, and D. W. Roberts, Sci. Rep. 2, 798 (2012). doi 10.1038/srep00798

    Article  ADS  Google Scholar 

  8. Y. Xiea, M. Thomb, A. Miserocchic, A. W. McEvoyc, A. Desjardinsd, S. Ourselina, and T. Vercauterena, Proc. SPIE 10050, 1005002 (2017). doi 10.1117/ 12.2250159

    Google Scholar 

  9. G. Themelis, J. S. Yoo, K. S. Soh, R. Schulz, and V. Ntziachristos, J. Biomed. Opt. 14, 064012 (2009). doi 10.1117/1.3259362

    Article  ADS  Google Scholar 

  10. W. C. Lin, S. A. Toms, E. D. Jansen, and A. Mahadevan-Jansen, IEEE J. Sel. Top. Quant. Electron. 7, 996 (2001). doi 10.1109/2944.983305

    Article  ADS  Google Scholar 

  11. M. Sinaasappel and H. J. C. M. Sterenborg, Appl. Opt. 32, 541 (1993). doi 10.1364/AO.32.000541

    Article  ADS  Google Scholar 

  12. A. Bogaards, H. J. C. M. Sterenborg, J. Trachtenberg, B. C. Wilson, and L. Lilge, Lasers. Surg. Med. 39, 605 (2007). doi 10.1002/lsm.20525

    Article  Google Scholar 

  13. H. Xie, H. Liu, P. Svenmarker, J. Axelsson, C. T. Xu, S. Gräfe, J. H. Lundeman, H. P. H. Cheng, S. Svanberg, N. Bendsoe, P. E. Andersen, K. Svanberg, and S. Andersson-Engels, J. Biomed. Opt. 16, 066002 (2011). doi 10.1117/1.3585675

    Article  ADS  Google Scholar 

  14. Yu. P. Sinichkin, S. R. Utz, A. H. Mavliutov, and H. A. Pilipenko, J. Biomed. Opt. 3, 201 (1998). doi 10.1117/1.429876

    Article  ADS  Google Scholar 

  15. S. K. Chang, D. Arifler, R. Drezek, M. Follen, and R. Richards-Kortum, J. Biomed. Opt. 9, 511 (2004). doi 10.1117/1.1695559

    Article  ADS  Google Scholar 

  16. A. A. Kokhanovsky, J. Opt. Soc. Am. A 26, 1896 (2009). doi 10.1364/JOSAA.26.001896

    Article  ADS  Google Scholar 

  17. A. Kim, M. Khurana, Y. Moriyama, and B. C. Wilson, J. Biomed. Opt. 15, 067006 (2010). doi 10.1117/1.3523616

    Article  ADS  Google Scholar 

  18. D. A. Rogatkin and O. D. Smirnova, J. Opt. Technol. 80, 566 (2013).

    Article  Google Scholar 

  19. C. M. Gardner, S. L. Jacques, and A. J. Welch, Appl. Opt. 35, 1780 (1996). doi 10.1364/AO.35.001780

    Article  ADS  Google Scholar 

  20. D. Yudovsky and L. Pilon, Appl. Opt. 49, 6072 (2010). doi 10.1364/AO.49.006072

    Article  ADS  Google Scholar 

  21. R. B. Saager, D. J. Cuccia, S. Saggese, K. M. Kelly, and A. J. Durkin, J. Biomed. Opt. 16, 126013 (2011). doi 10.1117/1.3665440

    Article  ADS  Google Scholar 

  22. B. Yang, M. Sharma, and J. W. Tunnell, J. Biomed. Opt. 18, 080503 (2013). doi 10.1117/1.JBO.18.8.080503

    Article  ADS  Google Scholar 

  23. B. Yang and J. W. Tunnell, J. Biomed. Opt. 19, 090505 (2014). doi 10.1117/1.JBO.19.9.090505

    Article  ADS  Google Scholar 

  24. J. Kress, D. J. Rohrbach, K. A. Carter, D. Luo, S. Shao, S. Lele, J. F. Lovell, and U. Sunar, Biomed. Opt. Express 6, 3546 (2015). doi 10.1364/BOE.6.003546

    Article  Google Scholar 

  25. S. A. Lysenko, Opt. Spectrosc. 124, 129 (2018).

    Article  ADS  Google Scholar 

  26. S. A. Lisenko, J. Appl. Spectrosc. 84, 439 (2017). doi 10.1007/s10812-017-0489-0

    Article  ADS  Google Scholar 

  27. S. C. Kanick, S. C. Davis, Y. Zhao, T. Hasan, E. V. Maytin, B. W. Pogue, and M. S. Chapmand, J. Biomed. Opt. 19, 075002 (2014). doi 10.1117/1.JBO.19.7.075002

    Article  ADS  Google Scholar 

  28. S. A. Lisenko, V. A. Firago, M. M. Kugeiko, and A. I. Kubarko, J. Appl. Spectrosc. 83, 617 (2016). doi 10.1007/s10812-016-0337-7

    Article  ADS  Google Scholar 

  29. E. P. Zege, A. P. Ivanov, and I. L. Katsev, Image Transfer through a Scattering Medium (Minsk, Nauka Tekh., 1985; Springer, Berlin, Heidelberg, 1991).

    Book  Google Scholar 

  30. G. V. Rozenberg, Sov. Phys. Usp. 10, 188 (1967).

    Article  ADS  Google Scholar 

  31. M. J. C. van Gemert, S. L. Jacques, H. J. Sterenborg, and W. M. Star, EEE Trans. Biomed. Eng. 46, 1146 (1989). doi 10.1109/10.42108

    Article  Google Scholar 

  32. S. A. Lisenko and M. M. Kugeiko, Meas. Tech. 56, 721 (2013). doi 10.1007/s11018-013-0271-5

    Article  Google Scholar 

  33. S. A. Lysenko and M. M. Kugeiko, Meas. Tech. 56, 1302 (2014). doi 0.1007/s11018-014-0372-9

    Article  Google Scholar 

  34. S. A. Lisenko, M. M. Kugeiko, V. A. Firago, and A. N. Sobchuk, J. Appl. Spectrosc. 81, 118 (2014). doi 10.1007/s10812-014-9896-7

    Article  ADS  Google Scholar 

  35. R. L. P. van Veen, W. Verkruysse, and H. J. C. M. Ste-renborg, Opt. Lett. 27, 246 (2002). doi 10.1364/OL.27.000246

    Article  ADS  Google Scholar 

  36. P. R. Bargo, S. A. Prahl, T. T. Goodell, R. A. Sleven, G. Koval, G. Blair, and S. L. Jacques, J. Biomed. Opt. 10, 034018 (2005). doi 10.1117/1.1921907

    Article  ADS  Google Scholar 

  37. A. N. Bashkatov, E. A. Genina, and V. V. Tuchin, J. Innov. Opt. Health Sci. 4, 9 (2011). doi 10.1142/ S1793545811001319

    Article  Google Scholar 

  38. S. L. Jacques, Phys. Med. Biol. 58 (11), R37 (2013). doi 10.1088/0031-9155/58/11/R37

    Article  ADS  Google Scholar 

  39. A. J. Welch, C. Gardner, R. Richards-Kortum, E. Chan, G. Criswell, J. Pfefer, and S. Warren, Lasers Surg. Med. 21, 166 (1997).

    Article  Google Scholar 

  40. L. G. Henyey and J. L. Greenstein, Astrophys. J. 93, 70 (1941). doi 10.1086/144246

    Article  ADS  Google Scholar 

  41. A. N. Bashkatov, E. A. Genina, V. I. Kochubey, A. A. Gavrilova, S. V. Kapralov, V. A. Grishaev, and V. V. Tuchin, Med. Laser Appl. 22, 95 (2007). doi 10.1016/j.mla.2007.07.003

    Article  Google Scholar 

  42. K. R. Rollakanti, S. C. Kanick, S. C. Davis, B. W. Pogue, and E. V. Maytin, Photon. Lasers Med. 2, 287 (2013). doi 10.1515/plm-2013-0030

    Google Scholar 

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Authors and Affiliations

  1. Institute for Nature Management of the National Academy of Sciences of Belarus, Minsk, 220114, Belarus

    S. A. Lysenko

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Original Russian Text © S.A. Lysenko, 2018, published in Optika i Spektroskopiya, 2018, Vol. 125, No. 1, pp. 111–120.

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Lysenko, S.A. Correction of Fluorescent Images of Biological Tissues for the Effects of Light Absorption and Scattering. Opt. Spectrosc. 125, 113–122 (2018). https://doi.org/10.1134/S0030400X18070184

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