The interaction of semiconductor quantum dots and silver nanoparticles (AgNPs) with bacteriorhodopsin (BR), a membrane protein contained in the purple membrane (PM) of Halobacterium salinarum, is studied. It is shown that both types of nanoparticles are adsorbed efficiently on the surface of the purple membranes, modulating the parameters of the bacteriorhodopsin photocycle. Electrostatic interactions are found to be the main cause of the effect of nanoparticles on the bacteriorhodopsin photocycle. These results explain our earlier data on the “fixation” of the bacteriorhodopsin photocycle for protein molecules trapped after incubation of the purple membranes with silver nanoparticles near the location of the “hot spots” of the effect of surface-enhanced Raman scattering (SERS). It is demonstrated that exposure of silver nanoparticles with bacteriorhodopsin in SERS-active regions lowers the amount of bacteriorhodopsin molecules involved in phototransformations.
This is a preview of subscription content, log in to check access.
Buy single article
Instant access to the full article PDF.
Price includes VAT for USA
V. A. Oleinikov, K. E. Mochalov, D. O. Solovyeva, A. A. Chistyakov, E. P. Lukashev, and I. R. Nabiev, Opt. Spectrosc. 121, 210 (2016).
K. Mochalov, D. Solovyeva, A. Chistyakov, B. Zimka, E. Lukashev, I. Nabiev, and V. Oleinikov, Mater. Today Proc. 3, 502 (2016).
K. Mochalov, D. Solovyeva, A. Chistyakov, B. Zimka, E. Lukashev, I. Nabiev, and V. Oleinikov, Mater. Today Proc. 3, 497 (2016).
V. A. Krivenkov, D. O. Solovyeva, P. S. Samokhvalov, K. I. Brazhnik, G. E. Kotkovskiy, A. A. Chistyakov, E. P. Lukashev, and I. R. Nabiev, J. Phys.: Conf. Ser. 541, 012045 (2014).
K. Mavani and M. Shah, Int. J. Eng. Res. Technol. 2 (3), 1 (2013).
N. Bouchonville, A. le Cigne, A. Sukhanova, M. Molinari, and I. Nabiev, Laser Phys. Lett. 10, 085901 (2013).
A. Sukhanova, K. Even-Desrumeaux, A. Kisserli, T. Tabary, B. Reveil, J. M. Milot, P. Chames, D. Baty, M. Artemyev, V. Oleinikov, M. Pluot, J. H. M. Cohen, and I. Nabiev, Nanomed.: Nanotechnol. Biol. Med. 8, 516 (2012).
S. Y. Zaitsev, E. P. Lukashev, D. O. Solovyeva, A. A. Chistyakov, and V. A. Oleinikov, Colloids Surf. 117, 248 (2014).
N. V. Tkachenko, Optical Spectroscopy: Methods and Instrumentations (Elsevier, Amsterdam, 2006).
A. B. Rubin, Biophysics, The School-Book for Biological Special Higher Schools (Universitet, Moscow, 1999) [in Russian].
Original Russian Text © V.A. Oleinikov, E.P. Lukashev, S.Yu. Zaitsev, A.A. Chistyakov, D.O. Solovyeva, K.E. Mochalov, I. Nabiev, 2017, published in Optika i Spektroskopiya, 2017, Vol. 122, No. 1, pp. 36–41.
About this article
Cite this article
Oleinikov, V.A., Lukashev, E.P., Zaitsev, S.Y. et al. The effect of plasmon silver and exiton semiconductor nanoparticles on the bacteriorhodopsin photocycle in Halobacterium salinarum membranes. Opt. Spectrosc. 122, 30–35 (2017). https://doi.org/10.1134/S0030400X17010210