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The Drosophila Zinc Finger Proteins Aef1 and CG10543 Are Co-Localized with SAGA, SWI/SNF, and ORC Complexes on Gene Promoters and Involved in Transcription Regulation

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Abstract

In previous studies, we purified the DUB-module of the Drosophila SAGA complex and showed that a number of zinc proteins interact with it, including Aef1 and CG10543. In this work, we conducted a genome-wide study of the Aef1 and CG10543 proteins and showed that they are localized predominantly on the promoters of active genes. The binding sites of these proteins co-localize with the SAGA and dSWI/SNF chromatin modification and remodeling complexes, as well as with the ORC replication complex. It has been shown that the Aef1 and CG10543 proteins are involved in the regulation of the expression of some genes on the promoters of which they are located. Thus, the Aef1 and CG10543 proteins are new participants in the cell transcriptional network and co-localize with the main transcription and replication complexes of Drosophila.

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ACKNOWLEDGMENTS

This work was carried out using the equipment of the Shared Use Center of the Institute of Gene Biology, Russian Academy of Sciences.

Funding

This work was supported by the Russian Science Foundation, grant no. 20-14-00269.

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Authors and Affiliations

  1. Institute of Gene Biology, Russian Academy of Sciences, 119334, Moscow, Russia

    J. V. Nikolenko, N. E. Vorobyova & A. N. Krasnov

  2. Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, 119991, Moscow, Russia

    J. V. Nikolenko, M. M. Kurshakova, D. V. Kopytova & Y. A. Vdovina

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  1. J. V. Nikolenko
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  2. M. M. Kurshakova
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  3. D. V. Kopytova
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  4. Y. A. Vdovina
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  5. N. E. Vorobyova
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  6. A. N. Krasnov
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Correspondence to A. N. Krasnov.

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Nikolenko, J.V., Kurshakova, M.M., Kopytova, D.V. et al. The Drosophila Zinc Finger Proteins Aef1 and CG10543 Are Co-Localized with SAGA, SWI/SNF, and ORC Complexes on Gene Promoters and Involved in Transcription Regulation. Mol Biol 58, 700–707 (2024). https://doi.org/10.1134/S0026893324700286

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