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Spatial Reconstruction of TRPC Mechanoreceptors of the Ctenophore Mnemiopsis leidyi A. Agassiz, 1865

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Abstract—The ctenophore Mnemiopsis leidyi A. Agassiz, 1865 responds to gentle mechanical stimulus with intense luminescence; however, the mechanism of this phenomenon is unknown. We searched for possible mechanosensitive receptors that initiate signal transduction resulting in photoprotein luminescence. The three orthologous genes of mouse (5z96) and drosophila (5vkq) TRPC-proteins, such as ML234550a-PA (860 a.a.), ML03701a-PA (828 a.a.), and ML038011a-PA (1395 a.a.), were found in the M. leidyi genome. The latter protein contains a long ankyrin helix consisting of 16 ANK domains. Study of the annotated domains and the network of interactions between the interactome proteins suggests that the ML234550a-PA and ML03701a-PA proteins carry out cytoplasmic transduction, but ML038011a-PA provides intranuclear transduction of mechanical signals. Spatial reconstruction of the studied proteins revealed differences in their structure, which may be related to various functions of these proteins in the cell. The question of which of these proteins is involved in the initiation of luminescence after mechanical stimulation is discussed.

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ACKNOWLEDGMENTS

The authors thank M.V. Fadeeva and E.V. Sergeeva for help in the work, O.V. Mashukova, B.E. Anninsky, S.A. Tsarin, and O.S. Zav’yalova for support, K.A. Rybakova, V.D. Ratnikov, E.V. Motsar and M.I. Silakov for discussion of the manuscript and recommendation, and also to anonymous reviewers for helpful comments that allowed us to significantly improve the manuscript.

Funding

The work was carried out within the framework of the State Grand for the FRC IBSS, No. 0828-2018-0002.

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Authors and Affiliations

  1. Kovalevsky Institute of Biology of the Southern Seas, Russian Academy of Sciences, 299011, Sevastopol, Russia

    A. V. Kuznetsov

  2. Sevastopol State University, 299053, Sevastopol, Russia

    A. V. Kuznetsov

  3. Semenov Federal Research Center for Chemical Physics, Russian Academy of Sciences, 119991, Moscow, Russia

    D. N. Vtyurina

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Correspondence to D. N. Vtyurina.

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The authors declare that they have no conflicts of interest. This article does not contain a description of any research involving humans or highly organized animals as subjects.

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Abbreviations: a.a., amino acid residues (with a digit); TRP, transient receptor potential; TRPC, (C, canonical); TRPA (A, ankyrin); TRPN (N, no mechanoreceptor potential C); TRPM (M, melastatin); TRPS (S, soromelastatin); TRPV (V, vanilloid), TRPVL (VL, vanilloid-like); TRPML (ML, mucolipin); TRPP (P, polycystic); ANK, ankyrin domain; AR-ankyrin repeat.

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Kuznetsov, A.V., Vtyurina, D.N. Spatial Reconstruction of TRPC Mechanoreceptors of the Ctenophore Mnemiopsis leidyi A. Agassiz, 1865. Mol Biol 57, 755–763 (2023). https://doi.org/10.1134/S0026893323040106

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