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KLF6 Super-enhancer Regulates Cell Proliferation by Recruiting GATA2 and SOX10 in Human Hepatoma Cells

  • MOLECULAR CELL BIOLOGY
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Abstract

Super-enhancer consists of a large cluster of transcription enhancers that regulates the expression of genes playing an important role in the growth and development of malignant tumors. Recently, several attempts for the identification of super-enhancers have been made, but their functional role in tumor cells remains unclear. This paper aims at elucidating the functional properties of KLF6 super-enhancer related to the growth regulation of HepG2 cells, in relation to transcription factors (TFs). First, some TFs specifying KLF6 super enhancer were identified using CRISPR/Cas9 system and siRNA. Then, their effects on the expression of the target gene KLF6 were assessed. Last, their influence on the proliferation of tumor cells was considered using the MTT method. The study shows that the active enhancers of KLF6 super-enhancer recruit GATA2 and SOX10 TFs to control the expression of the target gene, KLF6. Our findings suggest that the activity of KLF6 super-enhancer is regulated by two TFs (GATA2 and SOX10), and its targeting may be a potential therapeutic strategy for the liver cancer therapy.

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ACKNOWLEDGMENTS

The authors gratefully acknowledge the support from University of Science of DPRK. The authors would also like to take the opportunity to express their heartfelt gratitude to all those who made contributions to the completion of this article.

Funding

This work was supported by National Natural Science Foundation of Korea (grant no. 54289562) and National Natural Science Foundation of China (grant no. 715773068).

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Authors and Affiliations

  1. Life Science Department, University of Science, 999095, Pyongyang, Democratic People’s Republic of Korea

    Kum Chol Ri, Myong Ryong Ri, Ju Hua Ri, Ji Hyon Kim & Jong Ho Ri

  2. School of Management, Harbin Institute of Technology, 150001, Harbin, Heilongjiang, China

    Kum Chol Ri

  3. Department of Engineering Machine, Pyongyang University of Mechanical Engineering, 950003, Pyongyang, Democratic People’s Republic of Korea

    Kwang Hun Kim

  4. Life Science Department, Kim Il Sung University, 999093, Pyongyang, Democratic People’s Republic of Korea

    Sun Il Choe

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Kum Chol Ri, Ri, M.R., Kim, K.H. et al. KLF6 Super-enhancer Regulates Cell Proliferation by Recruiting GATA2 and SOX10 in Human Hepatoma Cells. Mol Biol 56, 443–452 (2022). https://doi.org/10.1134/S0026893322030116

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