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Mesenchymal Stem Cells Cultured in 3D System Inhibit Non-Small Cell Lung Cancer Cells through p38 MAPK and CXCR4/AKT Pathways by IL-24 Regulating

  • MOLECULAR CELL BIOLOGY
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Abstract

Non-small cell lung cancer (NSCLC) is prevalent worldwide and has a high mortality rate. Even if mesenchymal stem cells (MSCs) are suggested as cancer treatment, the studies of their effects on NSCLC cells contradict each other, mainly due to utilization of two-dimensional (2D) culture system. Three-dimensional (3D) culture systems resemble tissue organization in vivo. Here we comprehensively explore the inhibitory effects of MSCs on NSCLC cells in a 3D culture system. We confirmed that the inhibitory effects of 3D-cultured MSCs (3D-MSCs) on the proliferation and migration of NSCLC cells are greater than that of the 2D-cultured MSCs. The expression of IL-24 in 3D-MSCs is higher than 2D-MSCs, which is the key factor to enhance the anti-tumor effect of MSCs. In these cells, IL-24 affects p38 MAPK and CXCR4/AKT pathways. Overall, this study provides the support for use of MSCs in tumor therapy.

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Funding

This work was supported by the National Natural Science Foundation of China (81201762).

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Authors and Affiliations

  1. College of Life Sciences and Bioengineering, Beijing Jiaotong University, 100044, Beijing, PR China

    F. Suo, M. Pan, Y. Li, Q. Yan, H. Hu & L. Hou

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Correspondence to L. Hou.

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Suo, F., Pan, M., Li, Y. et al. Mesenchymal Stem Cells Cultured in 3D System Inhibit Non-Small Cell Lung Cancer Cells through p38 MAPK and CXCR4/AKT Pathways by IL-24 Regulating. Mol Biol 55, 589–603 (2021). https://doi.org/10.1134/S0026893321030110

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