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The SAGA Deubiquitinilation (DUB) Module Participates in Pol III-Dependent Transcription

  • MOLECULAR CELL BIOLOGY
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Abstract

SAGA, the multicomponent complex responsible for acetylation of histone N-terminal lysine residues, is involved in the transcription activation of a wide range of eukaryote genes. SAGA contains a protein module, DUB, which is responsible for histone deubiquitination. In this paper we show that the DUB module may be found within cells independently of SAGA. In the absence of SAGA, the DUB module may be recruited to the promoters of Pol III-transcribed genes, but not to the Pol II-dependent promoters. The DUB module is required to recruit transcription factor Brf1, a subunit of the Pol III-recruiting TFIIIB complex, to the promoters of Pol III-dependent genes. The DUB-module interacts with Pol III in vivo. The DUB-module is essential for recruiting both TFIIIB complexes and PBP complexes to the promoters of Pol III-dependent genes.

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ACKNOWLEDGMENTS

The work was performed using the equipment of the Center for Collective Use of the IBG RAS. The authors are grateful to R.Kh. Ziganshin. for the MALDI-TOF MS analysis performed on the equipment of the Shemyakin and Ovchinnikov Institute of Bioorganic Chemistry RAS.

Funding

This work was supported by grants from the Russian Foundation for Basic Research (no. 18-04-00514 A, D.K., E.N., A.G.) and the Russian Science Foundation (no. 20-14-00269, Yu.N., Yu.V.).

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Authors and Affiliations

  1. Institute of Gene Biology, Russian Academy of Sciences, 119334, Moscow, Russia

    J. V. Nikolenko, Y. A. Vdovina, E. I. Fefelova, A. A. Glukhova, E. N. Nabirochkina & D. V. Kopytova

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  1. J. V. Nikolenko
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  2. Y. A. Vdovina
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  3. E. I. Fefelova
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  4. A. A. Glukhova
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  5. E. N. Nabirochkina
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  6. D. V. Kopytova
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Correspondence to D. V. Kopytova.

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Additional information

Abbreviations: Pol II, RNA polymerase II; Pol III, RNA polymerase III; DUB-module, deubiquitination module; HAT-module, acetyltransferase module; snRNA, small nuclear RNA; PSE, proximal sequence element; DSE, distal sequence element; PSE-binding protein complex, a complex that binds the proximal element.

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Nikolenko, J.V., Vdovina, Y.A., Fefelova, E.I. et al. The SAGA Deubiquitinilation (DUB) Module Participates in Pol III-Dependent Transcription. Mol Biol 55, 432–440 (2021). https://doi.org/10.1134/S0026893321020278

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