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Unsolved Puzzles of Qβ Replicase

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Abstract

Qβ phage replicase has been the first RNA-directed RNA polymerase purified to homogeneity and intensively studied in vitro. In the mid-sixties, papers on Qβ and related replicases appeared in nearly every issue of the PNAS journal. By 1968, the mechanism of its action seemed to be almost completely understood. However, even now, a half of century later, a number of fundamental questions remains unanswered. How does the replicase manage to prevent the template and its complementary copy from annealing during the entire replication round? How does it recognize its templates? What is the function of the translation factors present in the replicase molecule? What is the mechanism the replicase uses to join (recombine) separate RNA molecules? Even the determination of the crystal structure of Qβ replicase did not help much. Certainly, there remains a lot to discover in the replication of Qβ phage, one of the smallest viruses known.

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  1. Institute of Protein Research of the Russian Academy of Sciences, 142290, Pushchino, Moscow oblast, Russia

    A. B. Chetverin, V. I. Ugarov & H. V. Chetverina

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This work was supported by the Russian Foundation for Basic Research (project no. 17-04-00597) and by the Program of the Presidium of the Russian Academy of Sciences for Basic Research in Molecular and Cell Biology and Postgenomic Technologies.

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The authors declare that they have no conflict of interest. This article does not contain any studies involving animals or human participants performed by any of the authors.

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A shorter version of this review has been presented at the International Conference “Viruses: Discovering Big in Small” held during March 11–12, 2019 in Moscow and dedicated to the 90th birthday of Professor Vadim Izrailevich Agol.

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Chetverin, A.B., Ugarov, V.I. & Chetverina, H.V. Unsolved Puzzles of Qβ Replicase. Mol Biol 53, 791–801 (2019). https://doi.org/10.1134/S0026893319060049

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