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Determination of proteomic and metabolic composition of exhaled breath condensate of newborns

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Abstract

Here, the possibility of proteomic and metabolomic analysis of the composition of exhaled breath condensate of neonates with respiratory support. The developed method allows non-invasive collecting sufficient amount of the material for identification of disease-specific biomarkers. Samples were collected by using a condensing device that was incorporated into the ventilation system. The collected condensate was analyzed by liquid chromatography coupled with high resolution mass spectrometry and tandem mass spectrometry. The isolated substances were identified with a use of databases for proteins and metabolites. As a result, a number of compounds that compose the exhaled breath condensate was determined and can be considered as possible biomarkers of newborn diseases or stage of development.

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Authors and Affiliations

  1. Kulakov Research Center for Obstetrics, Gynecology, and Perinatology, Moscow, 117997, Russia

    A. S. Kononikhin, V. V. Chagovets, N. L. Starodubtseva, A. Y. Ryndin, A. E. Bugrova, I. A. Popov, V. E. Frankevich, O. V. Ionov & G. T. Sukhikh

  2. Moscow Institute of Physics and Technology (State University), Dolgoprudnyi, Moscow oblast, 141700, Russia

    A. S. Kononikhin, N. L. Starodubtseva, Y. I. Kostyukevich, I. A. Popov & E. N. Nikolaev

  3. Emanuel Institute for Biochemical Physics, Moscow, 119934, Russia

    A. E. Bugrova, Y. I. Kostyukevich, I. A. Popov & E. N. Nikolaev

Authors
  1. A. S. Kononikhin
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  2. V. V. Chagovets
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  3. N. L. Starodubtseva
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  4. A. Y. Ryndin
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  5. A. E. Bugrova
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  6. Y. I. Kostyukevich
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  7. I. A. Popov
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  8. V. E. Frankevich
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  9. O. V. Ionov
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  10. G. T. Sukhikh
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  11. E. N. Nikolaev
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Corresponding author

Correspondence to A. S. Kononikhin.

Additional information

Original Russian Text © A.S. Kononikhin, V.V. Chagovets, N.L. Starodubtseva, A.Y. Ryndin, A.E. Bugrova, Y.I. Kostyukevich, I.A. Popov, V.E. Frankevich, O.V. Ionov, G.T. Sukhikh, E.N. Nikolaev, 2016, published in Molekulyarnaya Biologiya, 2016, Vol. 50, No. 3, pp. 540–544.

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Kononikhin, A.S., Chagovets, V.V., Starodubtseva, N.L. et al. Determination of proteomic and metabolic composition of exhaled breath condensate of newborns. Mol Biol 50, 470–473 (2016). https://doi.org/10.1134/S0026893316020096

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  • DOI: https://doi.org/10.1134/S0026893316020096

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