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Changes in the expression of dopaminergic genes in brain structures of male mice exposed to chronic social defeat Stress: An RNA-seq study

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Abstract

Whole-transcriptome analysis (RNA-seq) has been used to analyze changes in the expression of dopaminergic genes that encode proteins involved in the synthesis, inactivation, and neurotransmission of dopamine in the striatum, ventral tegmental area, raphe nuclei of the midbrain, hypothalamus, and hippocampus of male mice subjected to chronic social defeat. The expression of Th, Ddc, and Slc6A3 (Dat1) was upregulated, while that of Ppp1r1b and Sncg was downregulated in the ventral tegmental area; the expression of Th, Ddc, Drd2, and Sncg was downregulated in the raphe nuclei of midbrain; the expression of Th, Aldh2, and Ppp1r1b was upregulated, while that of Маоа was downregulated in the hypothalamus; Drd1 and Snca expression was downregulated and that of Sncb was upregulated in the striatum, and Sncb expression was upregulated in the hippocampus. There were no statistically significant changes in the expression of Comt, Maob, Drd3, Drd4, or Drd5 in the brain areas analyzed in stressed male mice (compared to control animals). Thus, the number of differentially expressed dopaminergic genes and the direction of expression changes in male mice experiencing chronic stress are specific to regions of the brain.

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Abbreviations

Dbh :

dopamine-β-hydroxylase gene

Comt :

catechol-O-methyltransferase gene

Маоа and Maob :

monoamine oxidase A and B genes, respectively

Drd3, Drd4, and Drd5 :

genes that encode dopamine receptors type 3, 4, and 5, respectively

Th :

tyrosine hydroxylase gene

Ddc :

DOPA decarboxylase gene

Slc6A3 (Dat1) :

dopamine transporter gene

Ppp1r1b :

regulatory subunit B of protein phosphatase 1

Aldh2:

aldehyde dehydrogenase 2 gene

Snca, Sncb, and Sncg :

synuclein α, β, and γ genes, respectively

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Authors and Affiliations

  1. Institute of Cytology and Genetics, Siberian Branch of the Russian Academy of Sciences, Novosibirsk, 630090, Russia

    I. L. Kovalenko, D. A. Smagin, A. G. Galyamina, Yu. L. Orlov & N. N. Kudryavtseva

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  1. I. L. Kovalenko
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  2. D. A. Smagin
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  3. A. G. Galyamina
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  4. Yu. L. Orlov
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  5. N. N. Kudryavtseva
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Corresponding author

Correspondence to N. N. Kudryavtseva.

Additional information

Original Russian Text © I.L. Kovalenko, D.A. Smagin, A.G. Galyamina, Yu.L. Orlov, N.N. Kudryavtseva, 2016, published in Molekulyarnaya Biologiya, 2016, Vol. 50, No. 1, pp. 184–187.

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Kovalenko, I.L., Smagin, D.A., Galyamina, A.G. et al. Changes in the expression of dopaminergic genes in brain structures of male mice exposed to chronic social defeat Stress: An RNA-seq study. Mol Biol 50, 161–163 (2016). https://doi.org/10.1134/S0026893316010088

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  • DOI: https://doi.org/10.1134/S0026893316010088

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