Molecular Biology

, Volume 48, Issue 3, pp 390–398 | Cite as

Involvement of serine/threonine protein kinases in the cold stress response in the cyanobacterium Synechocystis sp. PCC 6803: Functional characterization of SpkE protein kinase

  • A. A. ZorinaEmail author
  • V. S. Bedbenov
  • G. V. Novikova
  • V. B. Panichkin
  • D. A. Los’
Cell Molecular Biology


Stress responses of the unicellular cyanobacterium Synechocystis involve several regulatory systems, including two-component ones, and negative supercoiling of genomic DNA. The role of serine/threonine protein kinases (STPKs) in the cold response was studied in Synechocystis. A screening of a collection of STPK mutants identified four enzymes—SpkB, SpkD, SpkE, and SpkG—as possible transcriptional regulators at lower temperatures. A proteome analysis in a SpkE Synechocystis mutant implicated SpkE in the formation of the protein pattern. In vitro phosphorylation assays of recombinant SpkE confirmed that the STPK was functionally active and utilized basic proteins as preferable substrates.


Synechocystis proteome serine/threonine protein kinases phosphorylation cold stress 


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Copyright information

© Pleiades Publishing, Inc. 2014

Authors and Affiliations

  • A. A. Zorina
    • 1
    Email author
  • V. S. Bedbenov
    • 1
  • G. V. Novikova
    • 1
  • V. B. Panichkin
    • 1
  • D. A. Los’
    • 1
  1. 1.Timiryazev Institute of Plant PhysiologyRussian Academy of SciencesMoscowRussia

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