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Dynamics of aberrant methylation of functional groups of genes in progression of breast cancer

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Abstract

For the first time, the epigenetic status of breast benign proliferative processes, malignant breast tumors, and metastases to regional lymph nodes has been studied using the GoldenGate Cancer Panel I DNA methylation microarray (Illumina, United States). The functional groups of differentially methylated genes were identified in each set of samples. The aberrant methylation of genes that regulate cell proliferation and mobility was found in the samples of benign proliferative breast processes. The aberrant methylation of genes responsible for cell differentiation and proliferation, as well as protein phosphorylation and cell mobility, was observed in the samples of malignant breast tumors. The differential methylation of the genes that regulate cell adhesion, the formation of anatomical structures, angiogenesis, immune response, signal transduction, and protein phosphorylation were found in samples with metastases to regional lymph nodes compared to the unaltered breast epithelium. It was found that tissues that range from benign proliferative processes and metastases to regional lymph nodes were generally characterized by a relatively lower level of epigenetic variability compared to the tissues of the primary tumor.

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Abbreviations

BC:

breast cancer

BPP:

benign proliferative processes

RLN:

regional lymph nodes

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Authors and Affiliations

  1. Institute of Medical Genetics, Siberian Branch of the Russian Academy of Medical Sciences, Tomsk, 634050, Russia

    N. A. Skryabin, E. N. Tolmacheva & I. N. Lebedev

  2. Institute of Oncology, Siberian Branch of the Russian Academy of Medical Sciences, Tomsk, 634050, Russia

    M. V. Zavyalova, E. M. Slonimskaya & N. V. Cherdyntseva

  3. Siberian State Medical University, Tomsk, 634050, Russia

    I. N. Lebedev, M. V. Zavyalova, E. M. Slonimskaya & N. V. Cherdyntseva

Authors
  1. N. A. Skryabin
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  2. E. N. Tolmacheva
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  3. I. N. Lebedev
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  4. M. V. Zavyalova
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  5. E. M. Slonimskaya
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  6. N. V. Cherdyntseva
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Correspondence to N. A. Skryabin.

Additional information

Original Russian Text © N.A. Skryabin, E.N. Tolmacheva, I.N. Lebedev, M.V. Zavyalova, E.M. Slonimskaya, N.V. Cherdyntseva, 2013, published in Molekulyarnaya Biologiya, 2013, Vol. 47, No. 2, pp. 302–310.

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Skryabin, N.A., Tolmacheva, E.N., Lebedev, I.N. et al. Dynamics of aberrant methylation of functional groups of genes in progression of breast cancer. Mol Biol 47, 267–274 (2013). https://doi.org/10.1134/S0026893313020131

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  • DOI: https://doi.org/10.1134/S0026893313020131

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