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Regulation of multidrug resistance genes by transcription factors of the BltR subfamily

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Abstract

BltR protein is a MerR family transcription factor experimentally characterized in Bacillus subtilis. It activates the transcription of genes encoding the multidrug transporter Blt and spermine/spermidine acetyltransferase BltD. Comparative genomics methods were applied to BltR-dependent regulons in 25 bacterial genomes. The structure of the promoter regions of the regulated genes is typical of MerR family activators: their binding sites are located in long spacers between promoter boxes. The regulated genes usually form divergons with the regulator genes. The transcription factors under consideration regulate the transcription of multidrug transporter and spermine/spermidine acetyltransferase genes. These transporters can be either secondary or ATP-dependent. The conservative function of both types as multidrug transporters is confirmed by phylogenetic analysis.

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Abbreviations

MDR:

multiple drug resistance

ABC:

ATP Binding Cassette

MATE:

Multidrug and Toxic Compound Extrusion

MFS:

Major Facilitator Superfamily

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Authors and Affiliations

  1. Moscow Institute of Physics and Technology, Moscow, 117303, Russia

    I. A. Zharov

  2. Kharkevich Institute for Information Transmission Problems, Russian Academy of Sciences, Moscow, 127994, Russia

    I. A. Zharov, M. S. Gelfand & A. E. Kazakov

  3. Department of Bioengineering and Bioinformatics, Moscow State University, Moscow, 119992, Russia

    M. S. Gelfand

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  1. I. A. Zharov
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  2. M. S. Gelfand
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  3. A. E. Kazakov
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Correspondence to I. A. Zharov.

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Original Russian Text © I.A. Zharov, M.S. Gelfand, A.E. Kazakov, 2011, published in Molekulyarnaya Biologiya, 2011, Vol. 45, No. 4, pp. 715–723.

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Zharov, I.A., Gelfand, M.S. & Kazakov, A.E. Regulation of multidrug resistance genes by transcription factors of the BltR subfamily. Mol Biol 45, 658–666 (2011). https://doi.org/10.1134/S002689331103023X

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  • DOI: https://doi.org/10.1134/S002689331103023X

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