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Patterns of histone modifications across the chicken alfa-globin genes’ domain

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Abstract

Using native chromatin imunoprecipitation (N-ChIP) followed by TaqMan RT-PCR quantitative analysis, we have determined the profiles of histone acetylation and histone methylation within the α-globin gene domain before and after switching of embryonic globin gene expression. The results obtained do not support a supposition that the inactivation of the embryonic α-type globin gene π in the erythroid cells of the adult lineage is mediated via formation of an inactive chromatin domain. On the other hand, we have demonstrated that suppression of the gene π activity in erythroid cells of adult lineage correlates with decrease of the histone acetylation level within the embryonic subdomain of the α-globin gene domain.

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Authors and Affiliations

  1. Institute of Gene Biology, Russian Academy of Sciences, Moscow, 117334, Russia

    E. S. Yudinkova, A. A. Gavrilov & S. V. Razin

  2. Biological Department, Moscow State University, Moscow, 119992, Russia

    D. A. Bunina, S. V. Ulyanov & S. V. Razin

Authors
  1. E. S. Yudinkova
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  2. D. A. Bunina
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  3. S. V. Ulyanov
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  4. A. A. Gavrilov
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  5. S. V. Razin
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Correspondence to E. S. Yudinkova.

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Original Russian Text © E.S. Yudinkova, D.A. Bunina, S.V. Ulyanov, A.A. Gavrilov, S.V. Razin, 2011, published in Molekulyarnaya Biologiya, 2011, Vol. 45, No. 4, pp. 662–667.

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Yudinkova, E.S., Bunina, D.A., Ulyanov, S.V. et al. Patterns of histone modifications across the chicken alfa-globin genes’ domain. Mol Biol 45, 608–613 (2011). https://doi.org/10.1134/S0026893311030216

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  • DOI: https://doi.org/10.1134/S0026893311030216

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