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Escherichia coli Dam-methylase as a molecular tool for mapping binding sites of the yeast transcription factor Rpn4

  • Molecular Biology of the Cell
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Abstract

Rpn4p is a transcription factor responsible for coordinated regulation of proteasomal genes in Saccharomyces cerevisiae. There are data suggesting an involvement of this factor in regulation of many other genes that comprise more than one tenth part of the yeast genome. Traditional methods are inapplicable for mapping of Rpn4p binding sites because of their extremely low level. We have developed a model system using Dam-methylase of E. coli which allows us to detect interaction of Rpn4p with its target genes. In this system, we have shown that Rpn4p is recruited to proteasomal genes only through interactions with DNA.

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Abbreviations

PACE:

Proteasome-Associated Control Element

MACE:

MAG1-Associated Control Element

DamID:

Dam identification method

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Authors and Affiliations

  1. Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, Moscow, 119991, Russia

    D. S. Spasskaya, D. S. Karpov & V. L. Karpov

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  1. D. S. Spasskaya
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  2. D. S. Karpov
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  3. V. L. Karpov
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Correspondence to D. S. Spasskaya.

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Original Russian Text © D.S. Spasskaya, D.S. Karpov, V.L. Karpov, 2011, published in Molekulyarnaya Biologiya, 2011, Vol. 45, No. 4, pp. 642–651.

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Spasskaya, D.S., Karpov, D.S. & Karpov, V.L. Escherichia coli Dam-methylase as a molecular tool for mapping binding sites of the yeast transcription factor Rpn4. Mol Biol 45, 591–599 (2011). https://doi.org/10.1134/S0026893311030186

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  • DOI: https://doi.org/10.1134/S0026893311030186

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