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Identification of proteins overexpressed in malignant gastric tumors: Comparison of results obtained by 2DE and bioinformatic search

  • Proteomics
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Abstract

A comparison of protein expression profiles in intestinal and diffuse gastric tumors and normal tissues by 2D gel electrophoresis identified three proteins (SOD2, S100A6, and TXN) overexpressed in tumors. SOD2 and TXN were more frequently overexpressed in diffuse tumors than in intestinal ones. Based on the published data on comparative 2DE protein expression analysis, a control panel of eleven proteins overexpressed in gastric tumors was compiled. A bioinformatic search for the respective mRNAs in the Oncomine database (representing mRNA transcription levels in tumor vs. normal tissues) showed the agreement of proteomic and transcriptomic data for seven of 11 proteins.

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Abbreviations

GC:

gastric cancer

2DE:

two-dimensional gel electrophoresis

DB:

database

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Authors and Affiliations

  1. Tomsk Cancer Research Institute, Russian Academy of Medical Sciences, Siberian Branch, Tomsk, 634009, Russia

    E. S. Grigorieva, S. G. Afanas’ev, N. V. Cherdyntseva, S. A. Tuzikov & E. L. Choinzonov

  2. Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, Moscow, 119991, Russia

    Yu. A. Bukurova, G. S. Krasnov, V. L. Karpov, N. A. Lisitsyn & S. F. Beresten

  3. Siberian State Medical University, Tomsk, 634050, Russia

    S. G. Afanas’ev & E. L. Choinzonov

Authors
  1. E. S. Grigorieva
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  2. Yu. A. Bukurova
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  3. G. S. Krasnov
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  4. S. G. Afanas’ev
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  5. N. V. Cherdyntseva
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  6. S. A. Tuzikov
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  7. E. L. Choinzonov
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  8. V. L. Karpov
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  9. N. A. Lisitsyn
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  10. S. F. Beresten
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Corresponding author

Correspondence to E. S. Grigorieva.

Additional information

Original Russian Text © E.S. Grigorieva, Yu.A. Bukurova, G.S. Krasnov, S.G. Afanas’ev, N.V. Cherdyntseva, S.A. Tuzikov, E.L. Choinzonov, V.L. Karpov, N.A. Lisitsyn, S.F. Beresten, 2011, published in Molekulyarnaya Biologiya, 2011, Vol. 45, No. 4, pp. 738–743.

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Grigorieva, E.S., Bukurova, Y.A., Krasnov, G.S. et al. Identification of proteins overexpressed in malignant gastric tumors: Comparison of results obtained by 2DE and bioinformatic search. Mol Biol 45, 680–685 (2011). https://doi.org/10.1134/S0026893311030058

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  • DOI: https://doi.org/10.1134/S0026893311030058

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