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Isolation, sequence identification and expression profile of three novel genes Rab2A, Rab3A and Rab7A from black-boned sheep (Ovis aries)

  • Genomics. Transcriptomics. Proteomics
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Abstract

Complete coding sequences of three Black-boned sheep (Ovis aries) genes Rab2A, Rab3A and Rab7A were amplified using reverse transcription polymerase chain reaction (RT-PCR) based on the conserved sequence information of cattle or other mammals known to be highly homologous to sheep ESTs. The Black-boned sheep Rab2A gene encodes a protein of 226 amino acids which contains the conserved putative RabL2 domain and is highly homologous to the Rab2A proteins of seven other species—cattle (96%), human (83%), Sumatran orangutan (82%), rat (81%), mouse (80%), African clawed frog (72%) and zebrafish (71%). The Black-boned sheep Rab3A gene encodes a protein of 220 amino acids that contains the conserved putative Rab3 domain and is very similar to the Rab3A proteins of four species—cattle (99%), African clawed frog (99%), Western clawed frog (98%) and zebrafish (95%). And the Black-boned sheep Rab7A gene encodes a protein of 207 amino acids that contains the conserved putative Rab7 domain and has high homology with the Rab7A proteins of six other species—human (99%), dog (99%), Sumatran orangutan (99%), zebrafish (97%), rabbit (97%) and African clawed frog (96%). Analysis of the phylogenetic tree has demonstrated that the Black-boned sheep Rab2A, Rab3A and Rab7A proteins share a common ancestor and the tissue expression analysis has shown that the corresponding genes are expressed in a range of tissues including leg muscle, kidney, skin, longissimus dorsi muscle, spleen, heart and liver. Our experiment is the first to provide the primary foundation for a further insight into these three sheep genes.

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Authors and Affiliations

  1. Faculty of Animal Science and Technology, Yunnan Agricultural University, Kunming, 650201, China

    Y. D. He, D. M. Xi, L. Y. Yang, Y. W. Tan, Q. Liu, H. M. Mao & W. D. Deng

  2. Faculty of Foreign Languages, Yunnan Agricultural University, Kunming, 650201, China

    D. D. Liu

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  1. Y. D. He
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He, Y.D., Liu, D.D., Xi, D.M. et al. Isolation, sequence identification and expression profile of three novel genes Rab2A, Rab3A and Rab7A from black-boned sheep (Ovis aries). Mol Biol 44, 14–22 (2010). https://doi.org/10.1134/S0026893310010036

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  • DOI: https://doi.org/10.1134/S0026893310010036

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