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Isolation and functional analysis of cotton universal stress protein promoter in response to phytohormones and abiotic stresses

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Abstract

The 949 bp promoter fragment upstream from the translation initiation site of the GUSP gene encoding a universal stress protein was isolated from the genomic DNA of Gossypium arboreum. Some putative cis-acting elements involved in stress responses including E-box, ABRE, DPBF-box, and MYB-core elements were found in the promoter region. In an Agrobacterium-mediated transient expression assay, strong activation of the GUSP full promoter region occurred in tobacco leaves following dehydration, abscisic acid, salt, heavy metal, gibberellic acid and dark treatments. Deletion analysis of the promoter revealed that the dehydration, abscisic acid and salt responses were affected by the deletion between −208 and −949 bp and showed 2–4-fold induction. However, in response to dark, gibberellic acid and heavy metals the induction was only 2-fold. These findings further our understanding of the regulation of GUSP expression. This is an important study as no report of this universal stress protein promoter is available in literature.

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Abbreviations

ABA:

Abscisic acid

GA3:

Gibberellic acid

RACE:

Random amplification of cDNA ends

MRE:

Metal response element

GUSP:

Gossypium universal stress protein

bZIP:

basic region leucine zipper

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Authors and Affiliations

  1. Center of Excellence in Molecular Biology, University of the Punjab, 87-Canal Bank Road, Thokar Niaz Baig Lahore, 53700, Pakistan

    Muzna Zahur, Asma Maqbool, Muhammad Irfan, Muhammad Younas Khan Barozai, Bushra Rashid, Shiekh Riazuddin & Tayyab Husnain

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  1. Muzna Zahur
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  2. Asma Maqbool
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  3. Muhammad Irfan
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  4. Muhammad Younas Khan Barozai
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Correspondence to Tayyab Husnain.

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Zahur, M., Maqbool, A., Irfan, M. et al. Isolation and functional analysis of cotton universal stress protein promoter in response to phytohormones and abiotic stresses. Mol Biol 43, 578–585 (2009). https://doi.org/10.1134/S0026893309040086

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  • DOI: https://doi.org/10.1134/S0026893309040086

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