Abstract
Colorectal cancer is one of the most common malignancies in developed countries. Scarce clinical signs at the early stages of the disease and the lack of fast and sensitive diagnostic techniques based on the detection of tumor specific protein markers contribute greatly to the high mortality rate. The search for such markers is significantly complicated by the high levels of major structural and cytoskeletal proteins in normal and tumor tissues. Extraction with 0.2 M NaCl in the presence of the nonionic detergent NP-40 was performed to enrich the soluble protein fraction. This modification resulted in a considerably increased sensitivity of detection of minor proteins that may enter the circulation during carcinogenesis. The soluble protein profiles of the paired colon adenocarcinoma and normal tissue specimens were compared using 2D gel electrophoresis, which enabled the detection of 10 proteins whose levels in tumors were elevated at least 10-fold as compared to normal tissue. The proteins were identified by MALDI-TOF mass spectrometry, and two new protein markers of colon cancer, TAF9 and CISH, were discovered. Low levels of CISH synthesis in most normal human tissues and tumors other than colorectal cancer make it a prospective candidate diagnostic marker for this type of cancer.
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Jemal A., Siegel R., Ward E., Murray T., Xu J., Smigal C., Thun M.J. 2006. Cancer statistics. CA Cancer J. Clin. 56, 106–130.
American Cancer Society, http://www.cancer.org/docroot/home/index.asp
Crawford N.P., Colliver D.W., Galandiuk S. 2003. Tumor markers and colorectal cancer: Utility in management. J. Surg. Oncol. 84, 239–248.
Duffy M.J., van Dalen A., Haglund C., Hansson L., Klapdor R., Lamerz R., Nilsson O., Sturgeon C., Topolcan O. 2003. Clinical utility of biochemical markers in colorectal cancer: European Group on Tumour Markers (EGTM) guidelines. Eur. J. Cancer. 39, 718–727.
Polley A.C., Mulholland F., Pin C., Williams E.A., Bradburn D.M., Mills S.J., Mathers J.C., Johnson I.T. 2006. Proteomic analysis reveals field-wide changes in protein expression in the morphologically normal mucosa of patients with colorectal neoplasia. Cancer Res. 66, 6553–6562.
O’Farrell P.H. 1975. High resolution two-dimentional electrophoresis of proteins. J. Biol. Chem. 250, 4007–4021.
Laemmly U.K. 1970. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 227, 680–685.
Krasnov G.S., Oparina N.Yu., Hankin S.L., Mashkova T.D., Ershov A.N., Zatsepina O.G., Karpov V.L., Beresten S.F. 2008. Identification of proteins with altered expression in colorectal cancer by means of 2D proteomics. Mol. Biol. 43, 348–356.
Matrix Science, http://matrixscience.com
SAGE Genie, http://cgap.nci.nih.gov/SAGE
Chaurand P., DaGue B.B., Pearsall R.S., Threadgill D.W., Caprioli R.M. 2001. Profiling proteins from azoxymethane-induced colon tumors at the molecular level by matrix-assisted laser desorption/ionization mass spectrometry. Proteomics. 10, 1320–1326.
Ghavami S., Kerkhoff C., Los M., Hashemi M., Sorg C., Karami-Tehrani F. 2004. Mechanism of apoptosis induced by S100A8/A9 in colon cancer cell lines: The role of ROS and the effect of metal ions. J. Leukoc. Biol. 76, 169–175.
Wang G., Wang X., Wang S., Song H., Sun H., Yuan W., Cao B., Bai J., Fu S. 2008. Colorectal cancer progression correlates with upregulation of S100A11 expression in tumor tissues. Int. J. Colorectal Dis. 23, 675–682.
Melle C., Ernst G., Schimmel B., Bleul A., Mothes H., Kaufmann R., Settmacher U., von Eggeling F. 2006. Different expression of calgizzarin (S100A11) in normal colonic epithelium, adenoma and colorectal carcinoma. Int. J. Oncol. 28, 195–200.
Liu L., Zhao L., Zhang Y., Zhang Q., Ding Y. 2007. Proteomic analysis of Tiam1-mediated metastasis in colorectal cancer. Cell Biol. Int. 31, 805–814.
Melle C., Osterloh D., Ernst G., Schimmel B., Bleul A., von Eggeling F. 2005. Identification of proteins from colorectal cancer tissue by two-dimensional gel electrophoresis and SELDI mass spectrometry. Int. J. Mol. Med. 16, 11–17.
Vougas K., Gaitanarou E., Marinos E., Kittas C., Voloudakis-Baltatzis I.E. 2008. Two-dimensional electrophoresis and immunohistochemical study of calreticulin in colorectal adenocarcinoma and mirror biopsies. J. BUON. 13, 101–107.
Toquet C., Jarry A., Bou-Hanna C., Bach K., Denis M.G., Mosnier J.F., Laboisse C.L. 2007. Altered calreticulin expression in human colon cancer: maintenance of calreticulin expression is associated with mucinous differentiation. Oncol. Rep. 17, 1101–1107.
Eskinazi R., Thony B., Svoboda M., Robberecht P., Dassesse D., Heizmann C.W., van Laethem J.L., Resibois A. 1999. Overexpression of pterin-4a-carbinolamine dehydratase/dimerization cofactor of hepatocyte nuclear factor 1 in human colon cancer. Am. J. Pathol. 155, 1105–1113.
Yoshikawa R., Yanagi H., Hashimoto-Tamaoki T., Morinaga T., Nakano Y., Noda M., Fujiwara Y., Okamura H., Yamamura T. 2004. Gene expression in response to antitumour intervention by polysaccharide-K (PSK) in colorectal carcinoma cells. Oncol. Rep. 12, 1287–1293.
Alrawi S.J., Schiff M., Carroll R.E., Dayton M., Gibbs J.F., Kulavlat M., Tan D., Berman K., Stoler D.L., Anderson G.R. 2006. Aberrant crypt foci. Anticancer Res. 26, 107–119.
Shibata M., Takekawa M., Amano S. 1998. Increased serum concentrations of soluble tumor necrosis factor receptor I in noncachectic and cachectic patients with advanced gastric and colorectal cancer. Surg. Today. 28, 884–888.
Luo L., Chen W.D., Pretlow T.P. 2005. CpG island methylation in aberrant crypt foci and cancers from the same patients. Int. J. Cancer. 115, 747–751.
Kummola L., Hamalainen J.M., Kivela J., Kivela A.J., Saarnio J., Karttunen T., Parkkila S. 2005. Expression of a novel carbonic anhydrase, CA XIII, in normal and neoplastic colorectal mucosa. BMC Cancer. 5, 41.
Alfonso P., Nunez A., Madoz-Gurpide J., Lombardia L., Sanchez L., Casal J.I. 2005. Proteomic expression analysis of colorectal cancer by two-dimensional differential gel electrophoresis. Proteomics. 10, 2602–2611.
Friedman D.B., Hill S., Keller J.W., Merchant N.B., Levy S.E., Coffey R.J., Caprioli R.M. 2004. Proteome analysis of human colon cancer by two-dimensional difference gel electrophoresis and mass spectrometry. Proteomics. 4, 793–811.
Mazzanti R., Solazzo M., Fantappie O., Elfering, S., Pantaleo P., Bechi P., Cianchi F., Ettl A., Giulivi C. 2006. Differential expression proteomics of human colon cancer. Am. J. Physiol. Gastrointest. Liver Physiol. 290, 1329–1338.
Tan H.T., Zubaidah R.M., Tan S., Hooi S.C., Chung M.C. 2006. 2-D DIGE analysis of butyrate treated HCT-116 cells after enrichment with heparin affinity chromatography. J. Proteome Res. 5, 1098–1106.
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Original Russian Text © G.S. Krasnov, S.L. Khankin, Yu.A. Bukurova, O.G. Zatsepina, N.Yu. Oparina, D.G. Garbuz, A. N. Ershov, T.D. Mashkova, V.L. Karpov, S.F. Beresten, 2009, published in Molekulyarnaya Biologiya, 2009, Vol. 43, No. 4, pp. 610–615.
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Krasnov, G.S., Khankin, S.L., Bukurova, Y.A. et al. Proteomic expression analysis of human colorectal cancer: Identification of soluble overexpressed proteins. Mol Biol 43, 562–566 (2009). https://doi.org/10.1134/S0026893309040050
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DOI: https://doi.org/10.1134/S0026893309040050