Abstract
Nine mutant ribosomal proteins L1 from the bacterium Thermus thermophilus and archaeon Methanococcus jannaschii were obtained and their crystal structures were determined and analyzed. The structure of the S179C TthL1 mutant, determined earlier, was also analyzed. In half of the proteins studied, point mutations of the amino acid residues exposed on the protein surface essentially changed the spatial structure of the protein. This proves that a correct study of biological processes with the help of site-directed mutagenesis requires a preliminary determination or, at least, modeling of the structures of mutant proteins. A detailed comparison of the structures of the L1 mutants and the corresponding wild-type L1 proteins demonstrated that the side chain of a mutated amino acid residue tends to adopt a location similar to that of the side chain of the corresponding residue in the wild-type protein. This observation assists in modeling the structure of mutant proteins.
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References
Zimmermann R.A. 1980. Interactions among protein and RNA components of the ribosome. In: Ribosomes. Structure, Function and Genetics. Eds. Chambliss G., Craven G., Davies J., et al. Baltimore: University Park Press, pp. 135–169.
Gourse R.L., Sharrock R.A., Nomura M. 1986. Control of ribosome synthesis in Escherichia coli. In: Structure, Function, and Genetics of Ribosomes. Eds. Hardesty B., Kramer G. N.Y.: Springer, pp. 766–788.
Kraft A., Lutz C., Lingenhel A., Gröbner P., Piendl W. 1999. Control of ribosomal protein L1 synthesis in mesophilic and thermophilic archaea. Genetics. 152, 1363–1372.
Nikonov S., Nevskaya N., Eliseikina I., Fomenkova N., Nikulin A., Ossina N., Garber M., Jonsson B.-H., Briand C., Svensson A., Aevarsson A., Liljas A. 1996. Crystal structure of the RNA-binding ribosomal protein L1 from Thermus thermophilus. EMBO J. 15, 1350–1359.
Nevskaya N., Tishchenko S., Fedorov R., Al-Karadaghi S., Lilyas A., Kraft A., Piendl W., Garber M., Nikonov S. 2000. Archaeal ribosomal protein L1: The structure provides new insights into RNA binding of the L1 protein family. Structure. 8, 363–371.
Unge J., Al-Karadghi S., Liljas A., Jonsson B.-H., Eliseikina I., Ossina N., Nevskaya N., Fomenkova N., Garber M., Nikonov S. 1997. A mutant form of the ribosomal protein L1 reveals conformational flexibility. FEBS Lett. 411, 53–59.
Ho S.N., Hunt H.D., Horton R.M., Pullen J.K., Pease L.R. 1989. Site-directed mutagenesis by overlap extension using the polymerase chain reaction. Gene. 77, 51–59.
Calderone T.L., Stevens R.D., Oas T.G. 1996. High-level misincorporation of lysine for arginine at AGA codons in a fusion protein expressed in Escherichia coli. J. Mol. Biol. 262, 407–412.
Brinkmann U., Mattes R.E., Buckel P. 1989. High-level expression of recombinant genes in Escherichia coli is dependent on the availability of the dnaY gene product. Gene. 85, 109–114.
Kabsch W. 2001. Integration, scaling, space-group assignment and post refinement. In: International Tables for Crystallography. Eds. Rossmann M.G., Arnold E. Dordrecht: Kluwer, vol. F, chapter 25.2.9.
Storoni L.C., McCoy A.J., Read R.J. 2004. Likelihoodenhanced fast rotation functions. Acta Cryst. D60, 432–438.
Jones T.A., Zou J.Y., Cowan S.W., Kjeldgaard M. 1991. Improved methods for building protein models in electron density maps and the location of errors in these models. Acta Cryst. A47, 110–119.
Murshudov G.N., Vagin A.A., Dodson E.J. 1997. Refinement of macromolecular structures by the maximumlikelihood method. Acta Cryst. D57, 240–255.
Nevskaya N., Tishchenko S., Volchkov S., Kljashtorny V., Nikonova E., Nikonov O., Nikulin A., Köhrer C., Piendl W., Zimmermann R., Stockley P., Garber M., Nikonov S. 2006. New insights into the interaction of ribosomal protein L1 with RNA. J. Mol. Biol. 355, 747–759.
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Original Russian Text © E.Yu. Nikonova, S.A. Volchkov, V.G. Kljashtorny, S.V. Tishchenko, O.S. Kostareva, N.A. Nevskaya, O.S. Nikonov, A.G. Gabdoulkhakov, A.D. Nikulin, N.L. Davydova, V.A. Streltsov, M.B. Garber, S.V. Nikonov, 2007, published in Molekulyarnaya Biologiya, 2007, Vol. 41, No. 4, pp. 688–696.
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Nikonova, E.Y., Volchkov, S.A., Kljashtorny, V.G. et al. Crystal structures of mutant ribosomal proteins L1. Mol Biol 41, 622–629 (2007). https://doi.org/10.1134/S0026893307040152
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DOI: https://doi.org/10.1134/S0026893307040152