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Molecular Biology

, Volume 41, Issue 1, pp 70–76 | Cite as

Aberrant methylation of p16, HIC1, N33, and GSTP1 in tumor epithelium and tumor-associated cells in prostate cancer

  • T. V. Kekeeva
  • O. P. Popova
  • P. V. Shegai
  • B. Ya. Alekseev
  • Yu. Yu. Andreeva
  • D. V. Zaletaev
  • M. V. Nemtsova
Cell Molecular Biology

Abstract

The methylation status of p16, HIC1, N33, and GSTP1, which are involved in prostate carcinogenesis, was studied in prostate tissue samples containing neoplasms. Malignant acini, prostatic intraepithelial neoplasia (PIN) and benign prostatic hyperplasia (BPH) foci, and stroma surrounding glandular structures of each type were detected in histological sections, using laser capture microdissection of prostate tissue. High levels of methylation were found in tumor epithelium and adjacent tumor-associated stromal cells. Epigenetic changes in the stroma are indicative of a major role of tumor microenvironment in cancer development and progression. The methylation status of p16, HIC1, N33, and GSTP1 was also assessed in prostate biopsy material and operative tumor samples without laser capture microdissection. The methylation frequencies of all genes in tumor samples were considerably lower than those in microdissected tumor samples (HIC1, 71% vs. 89%; p16, 22% vs. 78%; GSTP1, 32% vs. 100%; and N33, 20% vs. 33%, respectively). It was concluded that laser capture micro-dissection is required in molecular analysis of tumors of this type.

Key words

prostate cancer methylation microdissection tumor microenvironment 

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Copyright information

© Pleiades Publishing, Inc. 2007

Authors and Affiliations

  • T. V. Kekeeva
    • 1
    • 2
  • O. P. Popova
    • 2
  • P. V. Shegai
    • 3
  • B. Ya. Alekseev
    • 3
  • Yu. Yu. Andreeva
    • 3
  • D. V. Zaletaev
    • 1
    • 2
  • M. V. Nemtsova
    • 1
    • 2
  1. 1.Medical Genetic Research CenterRussian Academy of Medical SciencesMoscowRussia
  2. 2.Institute of Molecular MedicineSechenov Moscow Medical Academy, Ministry of Health and Social Development of the Russian FederationMoscowRussia
  3. 3.Herzen Oncological Research InstituteMoscowRussia

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