Molecular Biology

, Volume 40, Issue 5, pp 758–763 | Cite as

Phenotypic expression of epigenetic determinant [ISP +] in Saccharomyces cerevisiae depends on the combination of sup35 and sup45 mutations

  • A. Yu. Aksenova
  • K. V. Volkov
  • N. S. Rovinsky
  • A. V. Svitin
  • L. N. Mironova
Genomics. Transcriptomics. Proteomics


Translation fidelity in Saccharomyces yeasts is determined by genetic and epigenetic (prion) factors. A study was made of S. cerevisiae strains containing the nonchromosomal determinant [ISP +], described earlier. Some of its properties suggest that [ISP +] is a prion. [ISP +] is expressed phenotypically as an antisuppressor of two sup35 mutations and can be cured with guanidine chloride (GuHCl). It was shown that sup35 mutants containing [ISP +] carried additional sup45 mutations. These mutations caused amino acid substitutions in different regions of translation termination factor eRF1, encoded by SUP45. Strains bearing the sup35-25 mutation contained the sup45 mutation that caused amino acid substitution at position 400 of eRF1; strains bearing sup35-10 contained the mutation that altered eRF1 at position 75. Thus, the antisuppressor phenotype of the [ISP +] strains proved to depend on the interaction of sup35 and sup45 mutations, as well as on the GuHCl-curable epigenetic determinant.

Key words

epigenetic inheritance yeast prions translation fidelity SUP45 SUP35 


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Copyright information

© Pleiades Publishing, Inc. 2006

Authors and Affiliations

  • A. Yu. Aksenova
    • 1
  • K. V. Volkov
    • 1
  • N. S. Rovinsky
    • 1
  • A. V. Svitin
    • 1
  • L. N. Mironova
    • 1
  1. 1.Department of Genetics and BreedingSt. Petersburg State UniversitySt. PetersburgRussia

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