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Characteristics of the interactions of cortisol-apolipoprotein A-I and tetrahydrocortisol-apolipoprotein A-I complexes with eukaryotic DNA

  • Molecular Mechanisms of Biological Processes
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Abstract

Primary hepatocyte culture has been used to demonstrate that the cortisol-apolipoprotein A-I complex does not affect the DNA and protein biosynthesis rates, whereas the tetrahydrocortisol-apolipoprotein A-I complex (THC-apoA-I) substantially increases the rates of 3H-thymidine and 14C-leucine incorporation into DNA and protein, respectively. Small-angle X-ray scattering data show that only THC-apoA-I effectively interacts with eukaryotic DNA, which is accompanied by local DNA melting. The (GCC)n repeat, a component of many human and other eukaryotic genes, is the most probable region of the interaction of this complex with DNA. An oligonucleotide (duplex) of this type has been synthesized. Its interaction with THC-apoA-I yields a larger complex, which breaks up, giving rise to complementary oligonucleotide strands. They also interact with THC-apoA-I. The equilibrium kinetics of this multiphase process is described. The interaction of the cortisol-apolipoprotein A-I complex with the duplex is less specific and does not cause its breakup or the formation of complementary oligonucleotides.

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Authors and Affiliations

  1. Institute of Biochemistry, Siberian Division, Russian Academy of Medical Sciences, Novosibirsk, 630117, Russia

    L. E. Panin & L. M. Polyakov

  2. Vector State Research Center of Virology and Biotechnology, Ministry of Health of the Russian Federation, Kol’tsovo, Novosibirsk oblast, 630559, Russia

    F. V. Tuzikov & N. A. Tuzikova

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  1. L. E. Panin
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  2. F. V. Tuzikov
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  3. N. A. Tuzikova
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  4. L. M. Polyakov
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Original Russian Text © L.E. Panin, F.V. Tuzikov, N.A. Tuzikova, L.M. Polyakov, 2006, published in Molekulyarnaya Biologiya, 2006, Vol. 40, No. 2, pp. 300–309.

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Panin, L.E., Tuzikov, F.V., Tuzikova, N.A. et al. Characteristics of the interactions of cortisol-apolipoprotein A-I and tetrahydrocortisol-apolipoprotein A-I complexes with eukaryotic DNA. Mol Biol 40, 261–269 (2006). https://doi.org/10.1134/S0026893306020129

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  • DOI: https://doi.org/10.1134/S0026893306020129

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