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Differentiation of capripoxvirus species and strains by polymerase chain reaction

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Abstract

A fast and simple method for capripoxvirus species identification has been developed. The method is based on multiplex polymerase chain reaction (MPCR) with species-specific primers and does not require nucleotide sequencing or restriction analysis of PCR products. To differentiate vaccine strains used in Russia and countries of the former Soviet Union from epizootic isolates of sheeppox virus, a method based on restriction analysis of the ankyrin-repeat protein gene fragment amplified by PCR has been developed. Being highly specific, both methods may be used for routine diagnosis of capripoxvirus-associated diseases.

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Authors and Affiliations

  1. Federal Center for Animal Health, Ministry of Agriculture of the Russian Federation, Vladimir, 600901, Russia

    E. S. Orlova, A. V. Shcherbakov, V. I. Diev & V. M. Zakharov

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  1. E. S. Orlova
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  2. A. V. Shcherbakov
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  3. V. I. Diev
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  4. V. M. Zakharov
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Original Russian Text © E.S. Orlova, A.V. Shcherbakov, V.I. Diev, V.M. Zakharov, 2006, published in Molekulyarnaya Biologiya, 2006, Vol. 40, No. 1, pp. 158–164.

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Orlova, E.S., Shcherbakov, A.V., Diev, V.I. et al. Differentiation of capripoxvirus species and strains by polymerase chain reaction. Mol Biol 40, 139–145 (2006). https://doi.org/10.1134/S0026893306010183

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  • DOI: https://doi.org/10.1134/S0026893306010183

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