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Effect of Cell-Free Culture Fluid of Staphylococcus aureus on the Structure and Biochemical Composition of Klebsiella pneumoniae and Pseudomonas aeruginosa Biofilms

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Abstract

Recently, there has been increasing evidence that many infections are associated with the formation of multispecies biofilms, in which there is a change in the sensitivity of bacteria to antibiotics and a change in the permeability of the extracellular matrix compared to monocultures. In this work, we show that the addition of cell-free culture liquid (CFCL) of Staphylococcus aureus to Klebsiella pneumoniae and Pseudomonas aeruginosa biofilms increases the content of α-, and β-polysaccharides in the matrix up to 2 times, which likely affects the structure of the biofilm. The increase in the polysaccharide component is also confirmed by a significant rise in the expression level of the K. pneumoniae pgaA and P. aeruginosa pelA, pslA genes in the presence of S. aureus culture liquid.

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Funding

The work was carried out with financial support from the Russian Science Foundation (project no. 20-64-47014).

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Authors and Affiliations

  1. Kazan (Volga Region) Federal University, 420008, Kazan, Russia

    A. V. Mironova, M. S. Fedorova, N. D. Zakarova, A. R. Salikhova, E. Y. Trizna & A. R. Kayumov

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  1. A. V. Mironova
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  2. M. S. Fedorova
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  3. N. D. Zakarova
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  4. A. R. Salikhova
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  5. E. Y. Trizna
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  6. A. R. Kayumov
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Contributions

M.A.V., F.M.S., Z.N.D., S.A.R.—experimental procedures, K.A.R., T.E.Yu.—work management; all authors contributed to the writing and approval of the manuscript.

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Correspondence to A. R. Kayumov.

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Mironova, A.V., Fedorova, M.S., Zakarova, N.D. et al. Effect of Cell-Free Culture Fluid of Staphylococcus aureus on the Structure and Biochemical Composition of Klebsiella pneumoniae and Pseudomonas aeruginosa Biofilms. Microbiology 93, 375–379 (2024). https://doi.org/10.1134/S0026261723604748

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  • DOI: https://doi.org/10.1134/S0026261723604748

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