Abstract
A total of 397 strains of Mycobacterium tuberculosis isolated from newly diagnosed patients with pulmonary tuberculosis in the Omsk oblast in 2019–2020 were genotyped. The prevalence of the Beijing genotype strains (70.8%), in particular, of two modern sublineage clusters: Central Asian/Russian (46.1%) and B0/W148 (19.1%), was established. Strains of the ancient sublineage of the Beijing genotype were represented by clusters 1071-32 and 14717-15, totaling 4.8%. Compared with other genotypes, the strains of B0/W148-cluster and the ancient Beijing sublineage were more often characterized by multidrug resistance (MDR): 93.4 and 94.7%, respectively (P < 0.0001). Among members of other genetic families (LAM, Ural, T, Haarlem), drug-sensitive strains predominated (75.0%). The circulation of MDR Beijing strains requires molecular epidemiological surveillance in view of their possible wider spread.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
REFERENCES
Kostyukova, I., Pasechnik, O., and Mokrousov, I., Epidemiology and drug resistance patterns of Mycobacterium tuberculosis in high-burden area in Western Siberia, Russia, Microorganisms, 2023, vol. 11, no. 2, p. 425. https://doi.org/10.3390/microorganisms11020425
Mokrousov, I., Vyazovaya, A., Pasechnik, O., Gerasimova, A., Dymova, M., Chernyaeva, E., Tatarintseva, M., and Stasenko, V., Early ancient sublineages of Mycobacterium tuberculosis Beijing genotype: unexpected clues from phylogenomics of the pathogen and human history, Clin. Microbiol. Infect., 2019, vol. 25, no. 8, pp. 1039.e1–1039.e6. https://doi.org/10.1016/j.cmi.2018.11.024
Mokrousov, I., Vyazovaya, A., Shitikov, E., Badleeva, M., Belopolskaya, O., Bespiatykh, D., Gerasimova, A., Ioannidis, P., Jiao, W., Khromova, P., Masharsky, A., Naizabayeva, D., Papaventsis, D., Pasechnik, O., Perdigão, J., et al., Insight into pathogenomics and phylogeography of hypervirulent and highly-lethal Mycobacterium tuberculosis strain cluster, BMC Infect. Dis., 2023, vol. 23, no. 1, p. 426. https://doi.org/10.1186/s12879-023-08413-7
Mokrousov, I., Vyazovaya, A., Sinkov, V., Gerasimova, A., Ioannidis, P., Jiao, W., Khromova, P., Papaventsis, D., Pasechnik, O., Perdigão, J., Rastogi, N., Shen, A., Skiba, Y., Solovieva, N., Suffys, P., et al., Practical approach to detection and surveillance of emerging highly resistant Mycobacterium tuberculosis Beijing 1071-32-cluster, Sci. Rep., 2021, vol. 11, no. 1, pp. 21392. https://doi.org/10.1038/s41598-021-00890-7
Pasechnik, O., Vyazovaya, A., Vitriv, S., Tatarintseva, M., Blokh, A., Stasenko, V., and Mokrousov, I., Major genotype families and epidemic clones of Mycobacterium tuberculosis in Omsk region, Western Siberia, Russia, marked by a high burden of tuberculosis–HIVcoinfection, Tuberculosis (Edinburgh), 2018, vol. 108, pp. 163–168. https://doi.org/10.1016/j.tube.2017.12.003
Roelens, M., Battista Migliori, G., Rozanova, L., Estill, J., Campbell, J.R., Cegielski, J.P., Tiberi, S., Palmero, D., Fox, G.J., Guglielmetti, L., Sotgiu, G., Brust, J.C.M., Bang, D., Lienhardt, C., Lange, C., et al., Evidence-based definition for extensively drug-resistant tuberculosis, Am. J. Respir. Crit. Care Med., 2021, vol. 204, no. 6, pp. 713–722. https://doi.org/10.1164/rccm.202009-3527OC
van Embden, J., Cave, M., Crawford, J., Dale, J.W., Eisenach, K.D., Gicquel, B., Hermans, P., Martin, C., McAdam, R., and Shinnick, T.M., Strain identification on Mycobacterium tuberculosis by DNA fingerprinting: recommendations for a standardized methodology, J. Clin. Microbiol., 1993, vol. 31, pp. 406–409.
Vyazovaya, A., Gerasimova, A., Mudarisova, R., Terentieva, D., Solovieva, N., Zhuravlev, V., and Mokrousov, I., Genetic diversity and primary drug resistance of Mycobacterium tuberculosis Beijing genotype strains in Northwestern Russia, Microorganisms, 2023, vol. 11, no. 2, p. 255. https://doi.org/10.3390/microorganisms11020255
Zhdanova, S., Mokrousov, I., Orlova, E., Sinkov, V., and Ogarkov, O., Transborder molecular analysis of drug-resistant tuberculosis in Mongolia and Eastern Siberia, Russia, Transbound. Emerg. Dis., 2022, vol. 69, no. 5, pp. e1800–e1814. https://doi.org/10.1111/tbed.14515
Funding
This study was supported by the Russian Science Foundation, project no. 19-14-00013.
Author information
Authors and Affiliations
Corresponding author
Ethics declarations
ETHICS APPROVAL AND CONSENT TO PARTICIPATE
This work does not contain any studies involving human and animal subjects.
CONFLICT OF INTEREST
The authors of this work declare that they have no conflict of interest.
Additional information
Translated by E. Babchenko
Publisher’s Note.
Pleiades Publishing remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.
Rights and permissions
About this article
Cite this article
Vyazovaya, A.A., Kostyukova, I.V., Gerasimova, A.A. et al. Drug Resistance of Different Mycobacterium tuberculosis Genotypes in the Omsk Oblast of Russia. Microbiology 93, 240–242 (2024). https://doi.org/10.1134/S002626172360444X
Received:
Revised:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1134/S002626172360444X