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Application of Flow Cytometry for Viability Assay of Mutants for Translation Termination Factors in the Yeast Saccharomyces cerevisiae

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Abstract

Nonsense mutations in the essential SUP45 and SUP35 genes encoding translation termination factors affect the viability of Saccharomyces cerevisiae cells. Flow cytometry revealed that the viability of the mutants was 3.5‒4 times lower compared to the wild-type strains. Moreover, the mutants were found to have higher sensitivity to ultrasonic treatment.

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ACKNOWLEDGMENTS

Equipment from the Resource Center “Development of Molecular and Cell Technologies” was used in the present study. This article is dedicated to the 300th anniversary of St. Petersburg State University.

Funding

This work was supported by the Russian Science Foundation, grant no. 23-14-00063.

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Authors and Affiliations

  1. St. Petersburg State University, 199034, St. Petersburg, Russia

    E. P. Efremova, O. M. Zemlyanko & G. A. Zhouravleva

  2. Laboratory of Amyloid Biology, St. Petersburg State University, 199034, St. Petersburg, Russia

    O. M. Zemlyanko & G. A. Zhouravleva

Authors
  1. E. P. Efremova
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  2. O. M. Zemlyanko
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  3. G. A. Zhouravleva
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Corresponding author

Correspondence to G. A. Zhouravleva.

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Translated by A. Oleskin

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Efremova, E.P., Zemlyanko, O.M. & Zhouravleva, G.A. Application of Flow Cytometry for Viability Assay of Mutants for Translation Termination Factors in the Yeast Saccharomyces cerevisiae. Microbiology 93, 236–239 (2024). https://doi.org/10.1134/S0026261723604244

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