Abstract
We searched for genes whose deletion affects mitochondrial heteroplasmy in the yeast Saccharomyces cerevisiae. In order to do this, we crossed a yeast knockout collection pool (rho+) with a rho– strain containing a large deletion in its mtDNA. The resulting mixture of cells was plated onto selective media that made it possible to distinguish between diploid cells that had lost and retained wild-type mtDNA as a result of such crossing. From the resulting pools (initial one, cells that lose rho+ mtDNA, and those that retained it), we isolated DNA and determined the proportion of deletion strains using high-throughput sequencing. This made it possible to identify functional groups of genes that reduce the ability of yeast cells to maintain rho+ mtDNA in a state of heteroplasmy with rho– mtDNA.
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The work was conducted with the support of the Russian Science Foundation grant, project 22-14-00108.
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Abbreviations: GO—gene ontology; mtDNA—mitochondrial DNA; ORF—open reading frame; YKO—yeast knockout.
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Burlaka, A.A., Glagoleva, E.S., Kashko, N.D. et al. Genetic Screening using High-Throughput Barcode Sequencing of Saccharomyces cerevisiae Knockout Collection: Search for Regulators of mtDNA Intracellular Selection. Microbiology 92 (Suppl 1), S114–S117 (2023). https://doi.org/10.1134/S0026261723604001
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DOI: https://doi.org/10.1134/S0026261723604001