Abstract—
The specific enzymatic reaction of sulfur reduction to hydrogen sulfide catalyzed by the HydSL hydrogenase of T. bogorovii BBS has so far been observed only in native cells. In this work, we were able to detect the hydrogen-dependent reaction H2 + S0 → H2S + HS– + H+ in a cell homogenate of these bacteria. This was achieved by destroying the cells in an optimized buffer of high ionic strength. Further purification of the preparation led to a 28-fold increase in its specific activity.
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This work was supported by the Russian Science Foundation, project no. 19-14-00255P.
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Translated by D. Timchenko
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Khasimov, M.K., Petushkova, E.P., Serdyuk, O.P. et al. Sulfur Metabolism in Thiocapsa bogorovii BBS and the Role of HydSL Hydrogenase. Microbiology 92 (Suppl 1), S22–S24 (2023). https://doi.org/10.1134/S002626172360386X
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DOI: https://doi.org/10.1134/S002626172360386X