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Phosphonatase Operons of Organophosphonate-Degrading Soil Bacteria of the Genus Achromobacter

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Abstract—

Novel phosphonoacetaldehyde hydrolases (phosphonatases) were isolated from glyphosate-degrading bacteria Achromobacter spp., and their kinetic and molecular characteristics were studied comparatively. Based on MLST analysis of their nucleotide reductase genes, the strains under study were identified as A. aegrifaciens and A. insolitus. Nonspecific induction of phosphonatase was observed in the presence of glyphosate, whereas the natural substrate of the phosphonatase pathway had a negligible effect. Complete genome sequencing disclosed presence of a phosphonatase operon of unusual structure in both bacterial species. In addition to the phosphonatase gene phnX, it also included a gene of a LysR-like transcriptional regulator and two genes of novel proteins with putative functions.

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Funding

Genome sequencing and analysis, as well as studies of genes of novel oxidoreductases found within phosphonatese operons of strains A. aegrifaciens Km 11 and A. insolitus Kg 13 were supported by Russian Science Foundation grant 23-24-00152 “Novel oxidoreductases of organophoshonate-degrading soil bacteria”.

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Authors and Affiliations

  1. Skryabin Institute of Biochemistry and Physiology of Microorganisms, Pushchino Scientific Center for Biological Research, Russian Academy of Sciences, 142290, Pushchino, Russia

    D. O. Epiktetov, A. V. Sviridov, S. V. Tarlachkov, T. V. Shushkova & A. A. Leontievsky

  2. Branch of Shemyakin and Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, 142290, Pushchino, Russia

    S. V. Tarlachkov

Authors
  1. D. O. Epiktetov
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  2. A. V. Sviridov
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  3. S. V. Tarlachkov
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  4. T. V. Shushkova
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  5. A. A. Leontievsky
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Correspondence to D. O. Epiktetov.

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Translated by D. Timchenko

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Epiktetov, D.O., Sviridov, A.V., Tarlachkov, S.V. et al. Phosphonatase Operons of Organophosphonate-Degrading Soil Bacteria of the Genus Achromobacter. Microbiology 92 (Suppl 1), S45–S49 (2023). https://doi.org/10.1134/S0026261723603548

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