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Detection of the Mycobacterium tuberculosis S-Genotype and Clarification of the Position of Controversial Spoligotypes of the Euro-American Lineage

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Abstract—

The S genotype (first described in Sicily, hence the name) is a part of the Euro-American lineage of Mycobacterium tuberculosis. Here, we report a method for its detection based on PCR-RFLP of the Rv0557 gene. The method was optimized on strains with known whole genome sequences and further applied to screening the DNA collections from Russia, Bulgaria, China, Japan, and Vietnam. PCR-RFLP analysis confirmed the S genotype in strains with the spoligotypes SIT34 (ancestral to S) and SIT1253 (Yakutia strains). Strains from Bulgaria with abridged spoligoprofiles SIT4 and SIT125 were also assigned to the S genotype. The developed method provides for rapid detection of the M. tuberculosis S genotype and reliably resolves phylogenetic ambiguity of the disputed spoligotypes.

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Funding

The study was supported by the scientific research program “Molecular, Organismal, and Population Patterns of Formation of the Epidemic Process of Anthroponoses and Transmissiblee Infections in Northern Asia and Adjacent Territories,” no. 121022500179-0 and by the North: Territory of Sustainable Development Scientific and Educational Center.

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Authors and Affiliations

  1. St. Petersburg Pasteur Institute, 197101, St.-Petersburg, Russia

    I. V. Mokrousov

  2. Scientific Centre of the Family Health and Human Reproduction Problems, 664003, Irkutsk, Russia

    S. N. Zhdanova & O. B. Ogarkov

  3. The Stephan Angeloff Institute of Microbiology, Bulgarian Academy of Sciences, 1113, Sofia, Bulgaria

    V. Valcheva

  4. Andreev “Phthisiatry” Scientific and Practical Center, Republic of Sakha (Yakutia), 677000, Yakutsk, Russia

    G. I. Alekseeva, M. K. Vinokurova & N. E. Evdokimova

Authors
  1. I. V. Mokrousov
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  2. S. N. Zhdanova
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  3. V. Valcheva
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  4. G. I. Alekseeva
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  5. M. K. Vinokurova
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  6. N. E. Evdokimova
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  7. O. B. Ogarkov
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Corresponding author

Correspondence to I. V. Mokrousov.

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Translated by P. Sigalevich

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Mokrousov, I.V., Zhdanova, S.N., Valcheva, V. et al. Detection of the Mycobacterium tuberculosis S-Genotype and Clarification of the Position of Controversial Spoligotypes of the Euro-American Lineage. Microbiology 92 (Suppl 1), S25–S28 (2023). https://doi.org/10.1134/S0026261723603457

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  • DOI: https://doi.org/10.1134/S0026261723603457

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