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Optimization of Bioformation of Silver Nanoparticles Using Azospirillum brasilense Cultures

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Abstract—

Herein we present the results of optimization of silver nanoparticles biosynthesis using a nonpathogenic soil bacterium Azospirillum brasilense. The conditions and parameters for synthesis of biogenic nanoparticles using A. brasilense vegetative cells, culture liquid, and homogeneous polyphenol oxidase preparations were analyzed. We have revealed the ranging effect of the incubation time, the concentration of silver ions, pH of the reaction mixture, and the concentration of metabolites, towards the production of colloidal silver nanoparticles with specified geometric parameters, high colloidal stability and relatively good monodispersity. The size, shape, and relative yield of electron-dense nanostructures were determined by transmission electron microscopy and dynamic light scattering; extinction spectra were recorded by UV-vis spectrophotometry.

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Funding

The work was supported by the Russian Science Foundation, grant no. 23-24-00570.

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Authors and Affiliations

  1. Institute of Biochemistry and Physiology of Plants and Microorganisms, Saratov Scientific Center, Russian Academy of Sciences, 410049, Saratov, Russia

    M. A. Kupryashina, E. P. Vetchinkina, A. S. Abdrakhmanova, E. G. Ponomareva, T. E. Pylaev, V. A. Khanadeev & A. M. Burov

  2. Razumovsky Saratov State Medical University, 410012, Saratov, Russia

    M. A. Kupryashina & T. E. Pylaev

Authors
  1. M. A. Kupryashina
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  2. E. P. Vetchinkina
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  3. A. S. Abdrakhmanova
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  4. E. G. Ponomareva
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  5. T. E. Pylaev
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  6. V. A. Khanadeev
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  7. A. M. Burov
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Corresponding author

Correspondence to M. A. Kupryashina.

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Translated by P. Sigalevich

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Kupryashina, M.A., Vetchinkina, E.P., Abdrakhmanova, A.S. et al. Optimization of Bioformation of Silver Nanoparticles Using Azospirillum brasilense Cultures. Microbiology 92 (Suppl 1), S41–S44 (2023). https://doi.org/10.1134/S002626172360338X

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  • DOI: https://doi.org/10.1134/S002626172360338X

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