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Pelosinus baikalensis sp. nov., an Iron-Reducing Bacterium Isolated from a Cold Freshwater Lake

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Abstract

An obligately anaerobic bacterium, strain Bkl1T, was isolated from an enrichment culture of iron-reducing bacteria (IRB) obtained from a sample of the bottom sediments of the cold freshwater Lake Baikal (Russia). Cells of the strain were Gram-stain-negative, motile, spore-forming straight rods (0.6–0.7 × 2.0–7.0 µm) with a fermentative metabolism. Strain Bkl1T grew in the temperature range from 7 to 38°C (optimum 20°C) and at pH 7.0–9.5 (optimum pH 7.6). The novel isolate was capable to reduce ferric citrate (FC), anthraquinone-2,6-disulfonate (AQDS) and Cr(VI) in the presence of lactate as carbon source. Ferrihydrite was not reduced in the absence of AQDS. Based on its 16S rRNA gene sequence, strain Bkl1T was affiliated to the family Sporomusaceae and, more specifically, to the genus Pelosinus. The strain was most closely related to Pelosinus fermentans DSM 17108T (99.2%) and P. propionicus DSM 13327T (99.1%). Genome relatedness indexes revealed that the average nucleotide identity (ANI) and digital DNA-DNA hybridization (dDDH) values between strain Bkl1T and its closest phylogenomic relative (P. fermentans DSM 17108T) were 93.3 and 54.2%, respectively. The G + C content of the genome of strain Bkl1T was 39.1 mol % and its size was 5.32 Mb with 4939 protein-coding genes. The predominant fatty acids in cell walls were С15:1, С17:1, and С16:1. Based on the phylogenetic analyses and phenotypic differences between the novel isolate and type strains of the genus Pelosinus, strain Bkl1T (=VKM B-3511Т = JCM 39258T) is proposed to represent a novel species Pelosinus baikalensis sp. nov.

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ACKNOWLEDGMENTS

The authors are grateful to T.I. Zemskaya (Laboratory of Carbohydrate Microbiology, Limnological Institute, Siberian Branch, Russian Academy of Sciences) and to O.P. Dagurova (Institute of General and Experimental Biology, Siberian Branch, Russian Academy of Sciences) for providing Lake Baikal bottom sediments.

Funding

This work was supported by the Russian Science Foundation (project no. 22-24-00518).

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Authors and Affiliations

  1. Skryabin Institute of Biochemistry and Physiology of Microorganisms, Federal Research Center “Pushchino Scientific Center for Biological Research of the Russian Academy of Sciences”, 142290, Pushchino, Moscow oblast, Russia

    A. G. Zakharyuk, N. E. Suzina & V. A. Shcherbakova

  2. Gubkin University, 119991, Moscow, Russia

    D. S. Kopitsyn

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  1. A. G. Zakharyuk
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Correspondence to A. G. Zakharyuk.

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The authors declare that they have no conflict of interest. This article does not contain any studies involving animals or human participants performed by any of the authors.

DATA AVAILABILITY

The GenBank/EMBL/DDBJ accession number for the 16S rRNA gene sequence of strain Bkl1T is MW805760. The whole genome sequence of strain Bkl1T is available at the NCBI (www.ncbi.nlm.nih.gov.) under accession number NZ_JAJHJB000000000.

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Zakharyuk, A.G., Kopitsyn, D.S., Suzina, N.E. et al. Pelosinus baikalensis sp. nov., an Iron-Reducing Bacterium Isolated from a Cold Freshwater Lake. Microbiology 92, 137–145 (2023). https://doi.org/10.1134/S0026261722602913

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