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Genes of NAD+-Dependent Formate Dehydrogenases in Taxonomy of Aerobic Methylotrophic Bacteria of the Genus Ancylobacter

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Abstract—

Comparative phylogenetic analysis of NAD+-dependent formate dehydrogenases (NAD+-FDH) genes, which have been detected in all available genomes of methylotrophs of the genera Ancylobacter, Starkeya, and Angulomicrobium, as well as in other members of the family Xanthobacteraceae (Xanthobacter, Aquabacter, Azorhizobium), was carried out. The position of Xanthobacteraceae on the tree constructed based on comparison of NAD+−FDH amino acid sequences was found to correlate with the 16S rRNA gene-based phylogeny. The sequences of the NAD+−FDH proteins of the genera Ancylobacter, Starkeya, and Angulomi-crobium exhibited 87.8–98.3% identity, indicating that this protein is very conservative within this group of methylotrophs. For the first time, analysis of the NAD+−FDH functional genes is recommended as a supplementary criterion for interspecies differentiation between methylotrophic bacteria of the genus Ancylobacter.

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Funding

The work was supported by the Russian Federation Ministry of Science and Higher Education, project no. 075-15-2021-1051.

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Authors and Affiliations

  1. Skryabin Institute of Biochemistry and Physiology of Microorganisms, Pushchino Scientific Centre of Biological Research, Russian Academy of Sciences, 142290, Pushchino, Russia

    A. A. Chemodurova, A. S. Reshetnikov, N. V. Agafonova & N. V. Doronina

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  1. A. A. Chemodurova
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  2. A. S. Reshetnikov
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  3. N. V. Agafonova
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  4. N. V. Doronina
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All authors analyzed and discussed the data and participated in the preparation of the manuscript.

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Correspondence to N. V. Doronina.

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This article does not contain any studies involving animals or human participants performed by any of the authors.

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Chemodurova, A.A., Reshetnikov, A.S., Agafonova, N.V. et al. Genes of NAD+-Dependent Formate Dehydrogenases in Taxonomy of Aerobic Methylotrophic Bacteria of the Genus Ancylobacter. Microbiology 91, 834–838 (2022). https://doi.org/10.1134/S0026261722601932

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  • DOI: https://doi.org/10.1134/S0026261722601932

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