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Determination of the Promising Microalgal Strain for Bioremediation of the Aquaculture Wastewater

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Abstract—

Applicability of three strains of green microalgae (Chlorella vulgaris SP BB-2, Parachlorella kessleri, and Chlamydomonas reinhardtii Dangeard СС-124) for bioremediation (contaminants removal) of aquaculture wastewater was investigated. C. vulgaris strain SP BB-2 exhibited the highest growth rate and biomass accumulation. This strain was found to utilize nitrogen and phosphorus from wastewater for growth and development and showed high efficiency according to chemical oxygen demand (COD). Moreover, among the studied strains, C. vulgaris SP BB-2 produced biomass with the highest protein content, which may be recommended as a feed additive for the aquaculture. Analysis of the induction and rapid light curves of chlorophyll fluorescence revealed alterations in the energy storage photosynthetic processes of C. vulgaris SP BB-2 cells when grown on the aquaculture wastewater. Thus, high values of the maximum quantum yield of primary photochemistry in photosystem II (PSII) (FV/FM), quantum yield of electron transport (φEo), PSII performance index on absorption basis (PIABS), and the light utilization coefficient (α) were observed. C. vulgaris strain SP BB-2 may be recommended for wastewater treatment, while its highly sensitive parameters of chlorophyll fluorescence induction (PIABS, φEo) may be used as indicators of the state of the microalgae.

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Funding

The work was carried out within the framework of the Moscow State University Development Program “Future of the Planet and Global Environmental Changes” and was supported by the Russian Foundation for Basic Research, project no. 20-04-00465, and by the Russian Science Foundation, project no. 20-64-46018.

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Authors and Affiliations

  1. Al-Farabi Kazakh National University, 050040, Almaty, Kazakhstan

    N. R. Akmukhanova, B. K. Zayadan, A. K. Sadvakasova, M. M. Torekhanova & M. O. Bauenova

  2. Moscow State University, 119991, Moscow, Russia

    N. P. Timofeev, D. A. Todorenko & D. N. Matorin

Authors
  1. N. R. Akmukhanova
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  2. B. K. Zayadan
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  3. A. K. Sadvakasova
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  4. M. M. Torekhanova
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  5. N. P. Timofeev
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  6. M. O. Bauenova
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  7. D. A. Todorenko
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  8. D. N. Matorin
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Correspondence to D. N. Matorin.

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The authors declare that they have no conflicts of interest. This article does not contain any studies involving animals or human participants performed by any of the authors.

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Translated by P. Sigalevich

Abbreviations: RC, reaction center; PSII, photosystem II.

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Akmukhanova, N.R., Zayadan, B.K., Sadvakasova, A.K. et al. Determination of the Promising Microalgal Strain for Bioremediation of the Aquaculture Wastewater. Microbiology 91, 533–541 (2022). https://doi.org/10.1134/S0026261722601166

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  • DOI: https://doi.org/10.1134/S0026261722601166

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