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Cell Wall Glycopolymers as a Diagnostic Trait of Arthrobacter crystallopoietes

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Abstract

The composition and structure of the cell wall glycopolymers from Arthrobacter crystallopoietes VKM Ac-1107T (family Micrococcaceae, phylum Actinobacteria), previously assigned to the “A. globiformis” group based on the high similarity of 16S rRNA gene sequences and traditional chemotaxonomic markers were studied. Teichoic acid—1,3-poly(glycerol phosphate) substituted with β-glucose residues, and diglycosyl 1-phosphate polymer with -6)-α-D-GalpNAc-(1→6)-α-D-GlcpNAc-(1-P-repeating unit were identified by chemical and NMR spectroscopy methods. The results of phylogenomic (taxogenomic) analysis, viz. determination of the average amino acid identity (AAI) and the similarity of conserved proteins (POCP), indicate that A. crystallopoietes belongs to a new genus and the composition of the cell wall glycopolymers may serve as a diagnostic characteristic of this genus, which will be described on the basis of A. crystallopoietes.

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Funding

The study was supported by the Ministry of Science and Higher Education of the Russian Federation (Grant agreement no. 075-15-2021-1051), and in the framework of the Scientific Project No 121032300094-7 of the Lomonosov State University.

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Authors and Affiliations

  1. Faculty of Biology, Moscow State University, 119234, Moscow, Russia

    N. V. Potekhina & T. M. Tul’skaya

  2. All-Russian Collection of Microorganisms (VKM), Skryabin Institute of Biochemistry and Physiology of Microorganisms, Pushchino Scientific Center for Biological Research, Russian Academy of Sciences, 142290, Pushchino, Russia

    E. V. Ariskina & L. I. Evtushenko

  3. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, 119991, Moscow, Russia

    A. S. Shashkov

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  1. N. V. Potekhina
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  2. E. V. Ariskina
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Correspondence to N. V. Potekhina.

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Translated by E. Babchenko

Accepted abbreviations: HSQC, proton-detected heteronuclear single-quantum correlation; COZY, correlation spectroscopy; TOCSY, total correlation spectroscopy; ROESY, rotating-frame nuclear overhauser effect correlation spectroscopy; HMBC, heteronuclear multiple bond correlation through several bonds; δС, δН, δP are the chemical shifts of the 13C, 1H, and 31Р atoms, respectively; AAI, average amino acid identity; POCP, percentage of conserved proteins.

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Potekhina, N.V., Ariskina, E.V., Shashkov, A.S. et al. Cell Wall Glycopolymers as a Diagnostic Trait of Arthrobacter crystallopoietes. Microbiology 91, 259–266 (2022). https://doi.org/10.1134/S0026261722300051

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