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Pinoxaden Degradation Characteristics of Acinetobacter pittobacter and Prediction of Related Genes

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Abstract

Pinoxaden is applied for weed control in the field to improve crop yield per unit area. This study assessed the methods to improve residual pinoxaden degradation in the soil. In this study, 14 strains were isolated from soil samples with pinoxaden as the only carbon and nitrogen source, and strain T4P20-1 exhibited high levels of pinoxaden degradation. The effects of different pH values, inoculation amounts, and cultivation times on the growth and degradation rate of strain T4P20-1 were studied. The optimal growth conditions of strain T4P20-1 were determined, and the degradation rate reached 92.23%. The morphological characteristics of strain T4P20-1 were determined by optical microscopy. Strain T4P20-1 was identified as Acinetobacter pittobacter based on its physiological and biochemical characteristics and 16S rDNA homology analysis. Through gene sequencing, the whole genome of strain T4P20-1 and GO, KEGG, COG, CAZymes, and PHI database annotations were obtained. The genes related to herbicide degradation were predicted by gene functional annotations. Herbicides can affect the germination and growth of the sensitive crops, and a negative correlation was noted between the herbicide concentration and the seed germination rate, bud length, and root length of maize. Thus, the strains screened in this study exhibited remediation effects on soil contaminated by pinoxaden. This information provides a new method for pinoxaden degradation in the future. This method can increase crop yields and reduce food security risks.

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ACKNOWLEDGMENTS

This work was supported by the Key Laboratory of Molecular Biology and Key Laboratory of Microbiology, College of Heilongjiang Province.

Funding

This research was funded by the National Key Research and Development Program of China (grant number 2017YFD0201703), the Heilongjiang Natural Science Foundation Research Team Project (grant number TD2019C002), and the University Nursing Program for Young Scholars with Creative Talents in Heilongjiang Province (grant number UNPYSCT 2017119).

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Authors and Affiliations

  1. Engineering Research Center of Agricultural Microbiology Technology, Ministry of Education, Heilongjiang University, 150500, Harbin, China

    F. Yang, W. Zeng, Ch. Liu & H. Fu

  2. Heilongjiang Provincial Key Laboratory of Ecological Restoration and Resource Utilization for Cold Region, School of Life Sciences, Heilongjiang University, 150080, Harbin, China

    F. Yang, Y. Wei, C. Sun, M. Yuan, W. Zeng, Ch. Liu & H. Fu

Authors
  1. F. Yang
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  2. Y. Wei
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  3. C. Sun
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  4. M. Yuan
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  5. W. Zeng
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  6. Ch. Liu
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  7. H. Fu
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Contributions

Conceptualization: F.Y. and W.Z.; Methodology: F.Y. and C.L.; Software: C.S. and Y.W.; Validation: C.S., Y.W., C.L., and F.Y.; Formal analysis: F.Y., M.Y., and Y.W.; Investigation: C.L., H.F., and C.S.; Resources: C.L. and C.S.; Data curation: Y.W. and C.S.; Writing—original draft preparation: C.S. and F.Y.; Writing—review and editing: Y.W. and C.L.; Visualization: Y.W. and C.S.; Supervision: F.Y., H.F., and C.L.; Project administration: F.Y. and C.L.; Funding acquisition: F.Y. All authors have read and agreed to the published version of the manuscript.

Corresponding authors

Correspondence to Ch. Liu or H. Fu.

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The authors declare that they have no conflicts of interest.

This article does not contain any studies involving animals or human participants performed by any of the authors.

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Yang, F., Wei, Y., Sun, C. et al. Pinoxaden Degradation Characteristics of Acinetobacter pittobacter and Prediction of Related Genes. Microbiology 91, 818–830 (2022). https://doi.org/10.1134/S002626172210109X

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  • DOI: https://doi.org/10.1134/S002626172210109X

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