Skip to main content

Phylogeny of Rhodotorula pinalis and Its Reclassification as Fellozyma pinalis comb. nov.

Abstract

The type strain Rhodotorula pinalis VKM Y-2963 has been characterized genetically and phylogenetically. The rDNA sequences obtained for the ITS region and D1/D2 domains of LSU have been deposited to NCBI GenBank (OM666053). Strains from the Moscow region, Fellozyma sp. KBP 3851 from Sphagnum mosses and Rh. pinalis VKM Y-2963 from dead conifer needles, were found to be conspecific. The results of phylogenetic analysis suggested that the species Rh. pinalis should be reassigned to the genus Fellozyma. The new combination, Fellozyma pinalis comb. nov., is proposed, MycoBank MB842988.

This is a preview of subscription content, access via your institution.

Fig. 1.

REFERENCES

  1. Boekhout, T., Aime, M.C., Begerow, D., Gabaldón, T., Heitman, J., Kemler, M., Khayhan, K., Lachance, M.-A., Louis, E.J., Sun, S., Vu, D., Yurkov, A., The evolving species concepts used for yeasts: from phenotypes and genomes to speciation networks, Fungal Diversity, 2021b, vol. 109, pp. 27–55.

    Article  Google Scholar 

  2. Boekhout, T., Amend, A.S., El Baidouri, F., Gabaldón, T., Geml, J., Mittelbach, M., Robert, V., Tan, C.S., Turchetti, B., Vu, D., Wang, Q.-M., and Yurkov, A., Trends in yeast diversity discovery, Fungal Diversity, 2021a. https://doi.org/10.1007/s13225-021-00494-6

  3. Fell, J.W., Boekhout, T., Fonseca, A., Scorzetti, G., and Statzell-Tallman, A., Biodiversity and systematics of basidiomycetous yeasts as determined by large-subunit rDNA D1/D2 domain sequence analysis, Int. J. Syst. Evol. Microbiol., 2000, vol. 50, pp. 1351–1371.

    CAS  Article  Google Scholar 

  4. Golubev, W.I., A new myo-inositol-positive species of the genus Rhodotorula, Mikol. Fitopatol., 2010, vol. 44, pp. 310–314.

    Google Scholar 

  5. Kachalkin, A.V., Glushakova, A.M., Yurkov, A.M., and Chernov, I.Yu., Characterization of yeast groupings in the phyllosphere of sphagnum mosses, Microbiology (Moscow), 2008, vol. 77, pp. 474–481.

    CAS  Article  Google Scholar 

  6. Katoh, K., Rozewicki, J., and Yamada, K.D., MAFFT online service: multiple sequence alignment, interactive sequence choice and visualization, Brief. Bioinform., 2019, vol. 20, pp. 1160–1166.

    CAS  Article  Google Scholar 

  7. Kurtzman, C.P. and Robnett, C.J., Identification and phylogeny of ascomycetous yeasts from analysis of nuclear large subunit (26S) ribosomal DNA partial sequences, Antonie van Leeuwenhoek, 1998, vol. 73, pp. 331–371.

    CAS  Article  Google Scholar 

  8. Kuznetsova, T.A., Vecherskii, M.V., Khayrullin, D.R., Stepankov, A.A., Maximova, I.A., Kachalkin, A.V., and Ushakova, N.A., Dramatic effect of black soldier fly larvae on fungal community in a compost, J. Sci. Food Agric., 2022, vol. 102, pp. 2598–2603. https://doi.org/10.1002/jsfa.11601

    CAS  Article  Google Scholar 

  9. Li, A.H., Yuan, F.X., Groenewald, M., Bensch, K., Yurkov, A.M., Li, K., Han, P.J., Guo, L.D., Aime, M.C., Sampaio, J.P., Jindamorakot, S., Turchetti, B., Inacio, J., Fungsin, B., Wang, Q.-M., and Bai, F.-Y., Diversity and phylogeny of basidiomycetous yeasts from plant leaves and soil: proposal of two new orders, three new families, eight new genera and one hundred and seven new species, Stud. Mycol., 2020, vol. 96, pp. 17–140.

    Article  Google Scholar 

  10. Liu, X.Z., Wang, Q.-M., Göker, M., Groenewald, M., Kachalkin, A.V., Lumbsch, H.T., Millanes, A.M., Wedin, M., Yurkov, A.M., Boekhout, T., and Bai, F.-Y., Towards an integrated phylogenetic classification of the Tremellomycetes, Stud. Mycol., 2015a, vol. 81, pp. 85–147.

    Article  Google Scholar 

  11. Liu, X.Z., Wang, Q.-M., Theelen, B., Groenewald, M., Bai, F.-Y., and Boekhout, T., Phylogeny of tremellomycetous yeasts and related dimorphic and filamentous basidiomycetes reconstructed from multiple gene sequence analyses, Stud. Mycol., 2015b, vol. 81, pp. 1‒26.

    CAS  Article  Google Scholar 

  12. Lücking, R., Aime, M.C., Robbertse, B., Miller, A.N., A-oki, T., Ariyawansa, H.A., Cardinali, G., Crous, P.W., Druzhinina, I.S., Geiser, D.M., Hawksworth, D.L., Hyde, K.D., Irinyi, L., Jeewon, R., Johnston, P.R., et al., Fungal taxonomy and sequence-based nomenclature, Nat. Microbiol., 2021, vol. 6, pp. 540–548.

    Article  Google Scholar 

  13. McNeill, J., Barrie, F., Buck, W., Demoulin, V., Greuter, W., Hawksworth, D., et al., International Code of Nomenclature for Algae, Fungi, and Plants (Melbourne Code), Königstein: Koeltz Scientific Books, 2012.

    Google Scholar 

  14. Schoch, C.L., Seifert, K.A, Huhndorf, S., Robert, V., Spouge, J.L., Levesque, C.A., Chen, W., and Fungal Barcoding Consortium, Nuclear ribosomal internal transcribed spacer (ITS) region as a universal DNA barcode marker for Fungi, Proc. Natl. Acad. Sci. U. S. A., 2012, vol. 109, pp. 6241–6246.

    CAS  Article  Google Scholar 

  15. Scorzetti, G., Fell, J.W., Fonseca, A., and Statzell-Tallman, A., Systematics of basidiomycetous yeasts: a comparison of large subunit D1/D2 and internal transcribed spacer rDNA regions, FEMS Yeast Res., 2002, vol. 2, pp. 495–517.

    CAS  Article  Google Scholar 

  16. Tamura, K., Stecher, G., Peterson, D., Filipski, A., and Kumar, S., MEGA6: Molecular Evolutionary Genetics Analysis version 6.0, Mol. Biol. Evol., 2013, vol. 30, pp. 2725–2729.

    CAS  Article  Google Scholar 

  17. Tedersoo, L., Bahram, M., Põlme, S., Kõljalg, U., Yorou, N.S., Wijesundera, R., Villarreal Ruiz, L., Vasco-Palacios, A.M., Thu, P.Q., Suija, A., Smith, M.E., Sharp, C., Saluveer, E., Saitta, A., Rosas, M., et al., Fungal biogeography. Global diversity and geography of soil fungi, Science, 2014, vol. 346, art. 1256688.

    Article  Google Scholar 

  18. Vu, D., Groenewald, M., Szöke, S., Cardinali, G., Eberhardt, U., Stielow, B., de Vries, M., Verkleij, G.J., Crous, P.W., Boekhout, T., and Robert, V., DNA barcoding analysis of more than 9000 yeast isolates contributes to quantitative thresholds for yeast species and genera delimitation, Stud. Mycol., 2016, vol. 85, pp. 91–105.

    CAS  Article  Google Scholar 

  19. Wang, Q.-M., Groenewald, M., Takashima, M., Theelen, B., Han, P.J., Liu, X.Z., Boekhout, T., and Bai, F.-Y., Phylogeny of yeasts and related filamentous fungi within Pucciniomycotina determined from multigene sequence analyses, Stud. Mycol., 2015b, vol. 81, pp. 27–53.

    Article  Google Scholar 

  20. Wang, Q.-M., Yurkov, A.M., Göker, M., Lumbsch, H.T., Leavitt, S.D., Groenewald, M., Theelen, B., Liu, X.Z., Boekhout, T., and Bai, F.-Y., Phylogenetic classification of yeasts and related taxa within Pucciniomycotina, Stud. Mycol., 2015a, vol. 81, pp. 149–189.

    Article  Google Scholar 

Download references

Funding

The study was supported by the Ministry of Science and Higher Education of the Russian Federation, agreement no. 075-15-2021-1051.

Author information

Authors and Affiliations

Authors

Corresponding author

Correspondence to A. V. Kachalkin.

Ethics declarations

The authors declare that they have no conflict of interest. This article does not contain any studies involving animals or human participants performed by any of the authors.

Additional information

Translated by E. Makeeva

Rights and permissions

Reprints and Permissions

About this article

Verify currency and authenticity via CrossMark

Cite this article

Kachalkin, A.V. Phylogeny of Rhodotorula pinalis and Its Reclassification as Fellozyma pinalis comb. nov.. Microbiology 91, 417–420 (2022). https://doi.org/10.1134/S0026261722100915

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1134/S0026261722100915

Keywords:

  • yeasts
  • taxonomy
  • Rhodotorula
  • Fellozyma
  • Chrysozymaceae
  • Pucciniomycotina