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Benzoate-Degrading Bacteria of the Family Halomonadaceae Isolated from a Salt Mining Area: Species Diversity and Analysis of the benA Genes

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Abstract—

Screening of ability to utilize benzoate as the sole carbon and energy source was carried out for 124 strains of the family Halomonadaceae (genera Halomonas, Chromohalobacter, Salinicola, and Kushneria) isolated from mining sites of the Upper Kama deposit of potassium and magnesium salts. Active growth on benzoate (in the presence of 30‒70 g/L NaCl) was shown for 28 Halomonas strains closely related to the species H. taeanensis, H. olivaria, H. ventosae, H. titanicae, H. alkaliantarctica, H. neptunia, H. radicis, and H. sulfidaeris. Strains of the genera Chromohalobacter, Salinicola, and Kushneria either did not grow on benzoate or carried out its transformation (two Chromohalobacter strains). PCR screening for the benA gene encoding the α-subunit of benzoate 1,2-dioxygenase (1,2-DO), the key enzyme for benzoate degradation, within the family Halomonadaceae revealed its presence in all benzoate-degrading Halomonas strains. The sequences of the amplified fragments had the highest similarity (not exceeding 95.50%) with the genes encoding the α-subunits of benzoate 1,2-DO, 2-chlorobenzoate 1,2-DO, and other dioxygenases of Halomonas strains containing Rieske-type [2Fe-2S] clusters. New data on the genetic systems regulating benzoate degradation in Halomonas isolates are of interest for better understanding of molecular mechanisms of aromatics degradation under salinization conditions. The isolated active benzoate degraders may be used to develop the technologies for bioremediation and monitoring of polluted soils.

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Funding

The study was performed according to State Assignment project no. АААА-А19-119112290008-4.

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  1. Institute of Ecology and Genetics of Microorganisms, Ural Branch, Russian Academy of Sciences, 614081, Perm, Russia

    A. A. Pyankova & E. G. Plotnikova

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  1. A. A. Pyankova
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Correspondence to E. G. Plotnikova.

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Translated by D. Timchenko

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Pyankova, A.A., Plotnikova, E.G. Benzoate-Degrading Bacteria of the Family Halomonadaceae Isolated from a Salt Mining Area: Species Diversity and Analysis of the benA Genes. Microbiology 91, 91–103 (2022). https://doi.org/10.1134/S0026261722010106

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