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Sphingomonas beigongshangi sp. nov., Isolated from Pit Mud of Baijiu

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Abstract

Two novel aerobic, gram-negative, rod-shaped bacterial strains designated strains REN5T and REN5-1, were isolated from pit mud of Baijiu in Sichuan province, China. Based on the 16S rRNA gene sequence similarity, both strains belonged to the genus Sphingomonas. Strains REN5T and REN5-1 showed the highest similarity with S. aquatilis (97.9–98.0%). The DNA G+C content of REN5T and REN5-1 were 67.62 and 67.57 mol %, respectively. The DNA-DNA relatedness values between strains REN5T and S. aquatilis, REN5-1 and S. aquatilis were 22.8 and 22.6%, respectively. The main isoprenoid quinone of REN5T was Q-10, the major fatty acids were C18:1ω7c, C16:0 and C14:0 2-OH, and the main are polar lipids were DPG (diphosphatidylglycerol), SGL (sphingoglycolipid), PG (phosphatidylglycerol), PC (phosphatidylcholine), PE (phosphatidylethanolamine), UPL (unidentified phospholipid) and UL (unidentified polar lipid). The polyphasic taxonomic data showed that strains REN5T and REN5-1 represented a novel species within the genus Sphingomonas, for which the name Sphingomonas beigongshangi sp. nov. is proposed, with the type strain REN5T (=GDMCC1.2211T = JCM 34404T).

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ACKNOWLEDGMENTS

We are grateful to Zou Xiaohui, associate researcher at National Institute for Viral Disease Control and Prevention, China CDC, with preparing transmission electron microscope.

Funding

This work supported by The National Key Research and Development Program of China (2018YFC1603606), Earmarked Fund for Hebei Edible Fungi Innovation Team of Modern Agro-Industry Technology Research System (HBCT2018050205), Science and Technology general project of Beijing municipal commission (KM202110011002), and Open fund project in Key Laboratory of Microbial Resources Collection and Preservation, Ministry of Agriculture and Rural Affairs (KLMRCP2021-03).

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Authors and Affiliations

  1. Key Laboratory of Brewing Molecular Engineering of China Light Industry, Beijing Technology and Business University, 100048, Beijing, China

    Zh. Sun, J. Xu, X. Xing, Y. Yan, Sh. Hao, J. Li & Q. Ren

  2. College of Horticulture, Hebei Agricultural University, 071001, Baoding, China

    Sh. Li

  3. Key Laboratory of Microbial Resources Collection and Preservation, Ministry of Agriculture and Rural Affairs, Chinese Academy of Agricultural Sciences, 100081, Beijing, China

    Zh. Sun

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AUTHOR CONTRIBUTIONS

Zh. Sun and J. Xu contributed equally to this work.

Conceptualization, Q.R. and S.M.L.; Formal analysis, Z.B.S., X.X. and J.L.; Funding acquisition, Q.R. and S.M.L.; Methodology, Z.B.S., J.L.X., X.X., Y.Y. and S.Y.H.; Writing—original draft, Z.B.S. and J.L.X.; Writing—review and editing, Q.R.

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Correspondence to Q. Ren or Sh. Li.

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The authors declare that there are no conflicts of interest. None of the authors performed experiments with animal or human participants.

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Sun, Z., Xu, J., Xing, X. et al. Sphingomonas beigongshangi sp. nov., Isolated from Pit Mud of Baijiu. Microbiology 92, 146–152 (2023). https://doi.org/10.1134/S0026261721101458

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