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Probiotic Attributes and Inhibitory Effects of Lactobacillus plantarum MYS84 against the Growth and Biofilm Formation of Pseudomonas aeruginosa

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Abstract

Manufacture of fermented foods/products, which, apart from basic nutrition, have health-promoting effects, is flourishing. Within the field of fermented foods, rapidly expanding is the area of probiotics. Probiotic bacteria are gaining growing attention in the last two decades as a result of the constantly increasing scientific evidence of their beneficial effects on human health. In the current study, the isolate Lactobacillus plantarum MYS84 was identified by morphological, biochemical, physiological, and genetic methods. The standard in vitro techniques revealed that the isolated strain possessed good probiotic attributes. The cell-free supernatant (CFS) was active against Pseudomonas aeruginosa MTCC 7903, probably due to its proteinaceous nature, and it retained activity after treatment with catalase, lysozyme, and papain. It was also heat-resistant and acid-stable. Moreover, the CFS exhibited strong biofilm inhibition (78%).

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Authors and Affiliations

  1. Department of Studies in Microbiology, University of Mysore, Mysuru-570006, Karnataka, Manasagangotri, India

    Poornachandra Rao K, Rakesh Somashekaraiah & M. Y. Sreenivasa

  2. Department of Botany, Yuvarajas College, University of Mysore, Mysuru-570005, Karnataka, India

    Hemanth Kumar N

  3. Department of Studies in Botany, University of Mysore, Mysuru-570006, Karnataka, Manasagangotri, India

    Hemanth Kumar N, M. Murali & Shobha J

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  1. Poornachandra Rao K
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  2. Hemanth Kumar N
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  3. Rakesh Somashekaraiah
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  4. M. Murali
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Correspondence to M. Y. Sreenivasa.

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Rao K, P., Kumar N, H., Somashekaraiah, R. et al. Probiotic Attributes and Inhibitory Effects of Lactobacillus plantarum MYS84 against the Growth and Biofilm Formation of Pseudomonas aeruginosa . Microbiology 90, 361–369 (2021). https://doi.org/10.1134/S0026261721030103

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