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A Novel Medium for Cultivation of “Desulforudis audaxviator”

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Abstract—

Slow-growing, hard-to-cultivate prokaryotes require special conditions for laboratory cultivation. The difficulty in cultivation may often prevent depositing the new isolates in the culture collections in order to validate their taxonomic status. The sulfate-reducing firmicute “Desulforudis audaxviator” strain BYF, has been isolated from a deep thermal borehole in West Siberia. In this study we showed that decreasing the concentrations of calcium and phosphate, as well as addition of a polyamine spermidine, increased the growth rate and biomass formation by these bacterium.

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Funding

The work was supported by the Russian Science Foundation, grant no. 18-14-00130.

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Authors and Affiliations

  1. Department of Plant Physiology, Biotechnology, and Bioinformatics, Tomsk State University, 634050, Tomsk, Russia

    A. P. Lukina & O. V. Karnachuk

Authors
  1. A. P. Lukina
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  2. O. V. Karnachuk
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Correspondence to O. V. Karnachuk.

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COMPLIANCE WITH ETHICAL STANDARDS

The authors declare that they have no conflict of interest. This article does not contain any studies involving animals or human participants performed by any of the authors.

AUTHOR CONTRIBUTION

A.P. Lukina carried out cultivation; O.V. Karnachuk planned the experiments and wrote the article.

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Translated by P. Sigalevich

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Lukina, A.P., Karnachuk, O.V. A Novel Medium for Cultivation of “Desulforudis audaxviator”. Microbiology 90, 401–404 (2021). https://doi.org/10.1134/S0026261721030073

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  • DOI: https://doi.org/10.1134/S0026261721030073

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