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Taxonomic Diversity and Size-Morphological Structure of Bacterioplankton of the Rybinsk Reservoir

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Abstract—

This is the first report on investigation of bacterioplankton taxonomic composition in the Rybinsk Reservoir by molecular biological methods. A total of 58 nucleotide sequences obtained were assigned to 26 operational taxonomic units (OTUs), which belonged to Eubacteria (24 OTUs) and Eukaryota (2 OTUs). The diversity of bacterioplankton was represented by seven phyla of eubacteria: Proteobacteria, Bacteroidetes, Verrucomicrobia, Firmicutes, Actinobacteria, Gemmatimonadetes, and Acidobacteria. Predominant organisms were alphaproteobacteria of the species “Candidatus Fonsibacter ubiquis,” as well as unidentified members of the phyla Bacteroidetes and Verrucomicrobia, whose homologues are widespread in freshwater environments and have small genomes and cells. During the study period, small and medium-sized free-living cells predominated in bacterioplankton (70–99%), because they were the least vulnerable to eliminating control factors. The majority of the bacterial community was formed by cocci (50–80%), while a significant part of its biomass was accounted for by small rods (up to 2 μm in length) (20–30%).

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ACKNOWLEDGMENTS

The authors are grateful to N.L. Bel’kova for her help in obtaining the results of molecular genetic analysis.

Funding

The work was carried out within the framework of the State Assignment no. АААА-А18-118012690098-5 and was supported by the Russian Foundation for Basic Research, project no. 15-34-50453 mol-nr.

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Authors and Affiliations

  1. Papanin Institute of Inland Water Biology, Russian Academy of Sciences, 152742, Borok, Russia

    E. V. Kuznetsova & D. B. Kosolapov

  2. Limnological Institute, Siberian Branch, Russian Academy of Sciences, 664033, Irkutsk, Russia

    E. V. Sukhanova

  3. Cherepovets State University, 162600, Cherepovets, Russia

    D. B. Kosolapov

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  1. E. V. Kuznetsova
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  2. E. V. Sukhanova
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Correspondence to E. V. Kuznetsova.

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The authors declare that they have no conflict of interest. This article does not contain any studies involving animals or human participants performed by any of the authors.

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Translated by P. Sigalevich

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Kuznetsova, E.V., Sukhanova, E.V. & Kosolapov, D.B. Taxonomic Diversity and Size-Morphological Structure of Bacterioplankton of the Rybinsk Reservoir. Microbiology 90, 324–335 (2021). https://doi.org/10.1134/S002626172103005X

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